The Tomato Metallocarboxypeptidase Inhibitor I, which Interacts with a Heavy Metal-Associated Isoprenylated Protein, Is Implicated in Plant Response to Cadmium
| Autor: | Barbara Molesini, Giovanni DalCorso, Antonella Furini, Elisa Fasani, Anna Manara, Federica Pennisi, Tiziana Pandolfini |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Arabidopsis thaliana metal ion binding proteins Pharmaceutical Science Carboxypeptidases Salt Stress 01 natural sciences Analytical Chemistry chemistry.chemical_compound Solanum lycopersicum Gene Expression Regulation Plant Drug Discovery Solanaceae abiotic stress heavy metals metallocarboxypeptidase inhibitors Abscisic acid Plant Proteins chemistry.chemical_classification 0303 health sciences biology food and beverages Biological activity Cell biology Chemistry (miscellaneous) Plant protein Molecular Medicine Cadmium Article lcsh:QD241-441 03 medical and health sciences lcsh:Organic chemistry Stress Physiological Endopeptidases Physical and Theoretical Chemistry 030304 developmental biology Abiotic stress Organic Chemistry fungi Compartmentalization (fire protection) biology.organism_classification Yeast Plant Leaves Enzyme chemistry 010606 plant biology & botany |
| Zdroj: | Molecules Volume 25 Issue 3 Molecules, Vol 25, Iss 3, p 700 (2020) |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules25030700 |
| Popis: | Metallocarboxypeptidases are metal-dependent enzymes, whose biological activity is regulated by inhibitors directed on the metal-containing active site. Some metallocarboxypeptidase inhibitors are induced under stress conditions and have a role in defense against pests. This paper is aimed at investigating the response of the tomato metallocarboxypeptidase inhibitor (TCMP)-1 to Cd and other abiotic stresses. To this aim, the tomato TCMP-1 was ectopically expressed in the model species Arabidopsis thaliana, and a yeast two-hybrid analysis was performed to identify interacting proteins. We demonstrate that TCMP-1 is responsive to Cd, NaCl, and abscisic acid (ABA) and interacts with the tomato heavy metal-associated isoprenylated plant protein (HIPP)26. A. thaliana plants overexpressing TCMP-1 accumulate lower amount of Cd in shoots, display an increased expression of AtHIPP26 in comparison with wild-type plants, and are characterized by a modulation in the expression of antioxidant enzymes. Overall, these results suggest a possible role for the TCMP-1/HIPP26 complex in Cd response and compartmentalization. |
| Databáze: | OpenAIRE |
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