Interpenetrating Hydrogel Networks Enhance Mechanical Stability, Rheological Properties, Release Behavior and Adhesiveness of Platelet-Rich Plasma

Autor: Piera Di Martino, Siyuan Deng, Cristina Casadidio, Roberta Censi, Fulvio Laus, Maria Giovanna Sabbieti, Dimitrios Agas, Maria Rosa Gigliobianco
Rok vydání: 2020
Předmět:
Cartilage
Articular
Platelet-derived growth factor
Polymers
Biocompatible Materials
02 engineering and technology
mechanical properties
Polyethylene Glycols
lcsh:Chemistry
chemistry.chemical_compound
Hyaluronic acid
Copolymer
Sulfones
Hyaluronic Acid
lcsh:QH301-705.5
Spectroscopy
chemistry.chemical_classification
Platelet-Rich Plasma
Temperature
Adhesiveness
Hydrogels
General Medicine
Polymer
Dynamic mechanical analysis
growth factor release
021001 nanoscience & nanotechnology
Computer Science Applications
Self-healing hydrogels
Intercellular Signaling Peptides and Proteins
Rheology
0210 nano-technology
interpenetrating polymer networks
rheological behavior
0206 medical engineering
Article
Catalysis
Inorganic Chemistry
Animals
Horses
tissue repair
Physical and Theoretical Chemistry
Molecular Biology
Acrylamides
Fibrin
Wound Healing
Organic Chemistry
thermosensitive hydrogels
020601 biomedical engineering
lcsh:Biology (General)
lcsh:QD1-999
chemistry
Platelet-rich plasma
Biophysics
Wound healing
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 4, p 1399 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 4
ISSN: 1422-0067
DOI: 10.3390/ijms21041399
Popis: Platelet‐rich plasma (PRP) has attracted much attention for the treatment of articular cartilagedefects or wounds due to its intrinsic content of growth factors relevant for tissue repair. However, the shortresidence time of PRP in vivo, due to the action of lytic enzymes, its weak mechanical properties and theconsequent short‐term release of bioactive factors has restricted its application and efficacy. The present workaimed at designing new formulation strategies for PRP, based on the use of platelet concentrate (PC)‐loadedhydrogels or interpenetrating polymer networks, directed at improving mechanical stability and sustainingthe release of bioactive growth factors over a prolonged time‐span. The interpenetrating hydrogels comprisedtwo polymer networks interlaced on a molecular scale: (a) a first covalent network of thermosensitive andbiodegradable vinyl sulfone bearing p(hydroxypropyl methacrylamide‐lacate)‐polyethylene glycol triblockcopolymers, tandem cross‐linked by thermal gelation and Michael addition when combined with thiolatedhyaluronic acid, and (b) a second network composed of cross‐linked fibrin. The PC‐loaded hydrogels, instead,was formed only by network (a). All the designed and successfully synthesized formulations greatlyincreased the stability of PRP in vitro, leading to significant increase in degradation time and storagemodulus of PRP gel. The resulting viscoelastic networks showed the ability to controllably release plateletderived growth factor and transforming growth factr &beta
1, and to improve the tissue adhesiveness of PRP. Thenewly developed hydrogels show great potential for application in the field of wound healing, cartilagerepair and beyond.
Databáze: OpenAIRE
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