Vitamin D sterols increase FGF23 expression by stimulating osteoblast and osteocyte maturation in CKD bone
| Autor: | Richard E. Bowen, Renata C. Pereira, Earl Freymiller, Katherine Wesseling-Perry, Isidro B. Salusky |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
musculoskeletal diseases Male medicine.medical_specialty Histology Adolescent Physiology Endocrinology Diabetes and Metabolism Osteoclasts 030209 endocrinology & metabolism Apoptosis Cell Count urologic and male genital diseases Osteocytes Article Bone and Bones 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Calcification Physiologic Osteogenesis Internal medicine Bone cell medicine Vitamin D and neurology Humans Doxercalciferol Renal Insufficiency Chronic Vitamin D Cells Cultured Osteoblasts Chemistry Osteoid Osteoblast Cell Differentiation In vitro maturation Fibroblast Growth Factors stomatognathic diseases Fibroblast Growth Factor-23 Sterols 030104 developmental biology medicine.anatomical_structure Endocrinology Osteocyte Ergocalciferols Sclerostin Female medicine.drug |
| Zdroj: | Bone |
| Popis: | Impaired osteoblast and osteocyte maturation contribute to mineralization defects and excess FGF23 expression in CKD bone. Vitamin D sterols decrease osteoid accumulation and increase FGF23 expression; these agents also increase osteoblast maturation in vitro but a link between changes in bone cell maturation, bone mineralization, and FGF23 expression in response to vitamin D sterols has not been established. We evaluated unmineralized osteoid accumulation, osteocyte maturity markers (FGF23:early osteocytes; sclerostin: late osteocytes), and osteocyte apoptosis in iliac crest of 11 pediatric dialysis patients before and after 8 months of doxercalciferol therapy. We then evaluated the effect of 1,25(OH)(2)vitamin D on in vitro maturation and mineralization of primary osteoblasts from dialysis patients. Unmineralized osteoid accumulation decreased while numbers of early (FGF23-expressing) increased in response to doxercalciferol. Osteocyte apoptosis was low but increased with doxercalciferol. Bone FGF23 expression correlated with numbers of early, FGF23-expressing, osteocytes (r=0.83, p |
| Databáze: | OpenAIRE |
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