Association between bacteria occurring in the apical canal system and expression of bone-resorbing mediators and matrix metalloproteinases in apical periodontitis
| Autor: | Isabel Schausltz Pereira Faustino, Flávio R.F. Alves, W. R. Araújo‐Filho, Cinthya Cristina Gomes, J. F. Siqueira, Ademar Takahama, Rebeca De Souza Azevedo, Isabela N. Rôças |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
Male musculoskeletal diseases 0301 basic medicine Pathology medicine.medical_specialty Root canal Biology Real-Time Polymerase Chain Reaction Lesion 03 medical and health sciences 0302 clinical medicine Tooth Apex Osteoprotegerin Dental Pulp Necrosis medicine Humans Parvimonas micra General Dentistry Aged Pulp necrosis Periodontitis Receptor Activator of Nuclear Factor-kappa B RANK Ligand Streptococcus 030206 dentistry Fusobacterium Middle Aged medicine.disease Matrix Metalloproteinases Exact test 030104 developmental biology medicine.anatomical_structure Matrix Metalloproteinase 9 RANKL biology.protein Matrix Metalloproteinase 2 Female Dental Pulp Cavity medicine.symptom Periapical Periodontitis |
| Zdroj: | International Endodontic Journal. 51:738-746 |
| ISSN: | 0143-2885 |
| DOI: | 10.1111/iej.12895 |
| Popis: | AIM To evaluate the association between the presence of selected bacterial species/groups in the apical root canal and expression of mediators of soft and bone tissue destruction in apical periodontitis lesions. Relationships between bacteria and some other features of apical periodontitis were also investigated. METHODOLOGY Seventeen freshly extracted teeth with pulp necrosis and apical periodontitis were included. The apical root segment was sectioned and cryopulverized; DNA was extracted and evaluated for the presence of 9 bacterial species/groups using real-time polymerase chain reaction. Lesions were processed for histopathological and immunohistochemical analyses, which targeted matrix metalloproteinase-2 (MMP-2) and -9 (MMP-9), receptor activator of NFκB (RANK), RANK ligand (RANKL) and osteoprotegerin (OPG). Associations of the target bacteria with expression of these mediators, presence of symptoms, lesion size and histopathological diagnosis were evaluated. Data were analysed using the chi-square, Fisher's exact, Mann-Whitney and Pearson tests. P values lower than 0.05 were considered significant. RESULTS All pulverized apical root samples were positive for bacteria. The most prevalent taxa were Actinobacteria (53%), Streptococcus species (35%), Fusobacterium species and Parvimonas micra (18%). The target mediators exhibited a high mean expression in the lesions (MMP-2: 82%; MMP-9: 73%; RANK: 78%; RANKL; 81%; OPG; 83%). Mean RANKL:OPG ratio was significantly higher in granulomas than cysts (P |
| Databáze: | OpenAIRE |
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