Hepatitis B virus (HBV)-specific cytotoxic T-cell (CTL) response in humans: characterization of HLA class II-restricted CTLs that recognize endogenously synthesized HBV envelope antigens
| Autor: | A. Cavalli, A Valli, Francis V. Chisari, P Fowler, Antonio Bertoletti, Robert F. Margolskee, Bernard Moss, S Guilhot, Franco Fiaccadori, Amalia Penna |
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| Rok vydání: | 1992 |
| Předmět: |
Hepatitis B virus
Immunology Vaccinia virus Cyclopentanes Human leukocyte antigen medicine.disease_cause Antiviral Agents Microbiology Ammonium Chloride Virus Immune system Viral Envelope Proteins Antigen Viral envelope Virology medicine Humans Cytotoxic T cell Antigens Viral Recombination Genetic HLA-D Antigens Brefeldin A biology Chloroquine Hepatitis B biology.organism_classification digestive system diseases Clone Cells Hepadnaviridae Insect Science CD4 Antigens Research Article T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of Virology. 66:1193-1198 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.66.2.1193-1198.1992 |
| Popis: | In this study, we show that CD4+, hepatitis B virus (HBV) envelope-specific T-cell clones produced by stimulation with a particulate antigen preparation are able to recognize and kill not only autologous antigen-presenting cells incubated with exogenous HBV envelope antigens but also autologous HLA class II-positive cells expressing endogenously synthesized HBV envelope antigens following infection with recombinant vaccinia viruses or transfection with recombinant Epstein-Barr virus expression vectors. Experiments with lysosomotropic agents and brefeldin A suggest that the endosomal compartment is likely involved in the processing of endogenously synthesized viral proteins for recognition by CD4+ T cells. Our study indicates that HBV envelope-specific, HLA class II-restricted CD4+ cytotoxic T lymphocytes can potentially participate in the immune clearance of HBV-infected cells and the pathogenesis of hepatocellular injury in hepatitis B. |
| Databáze: | OpenAIRE |
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