Studies on the Interaction between Three Small Flavonoid Molecules and Bovine Lactoferrin
| Autor: | Ziyu He, Zhenmin Liu, Bing Niu, Jun Yu, Xiaosheng Qu, Junyi Huang, Zhidian Niu, Liu Pan, Mengying Zhang, Qiaorong Ma |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Naringenin 030103 biophysics Article Subject Stereochemistry Flavonoid lcsh:Medicine 02 engineering and technology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences chemistry.chemical_compound Animals Molecule Flavonoids chemistry.chemical_classification Quenching (fluorescence) General Immunology and Microbiology Hydrogen bond lcsh:R General Medicine 021001 nanoscience & nanotechnology Binding constant Lactoferrin chemistry Cattle 0210 nano-technology Quercetin Luteolin Research Article Protein Binding |
| Zdroj: | BioMed Research International BioMed Research International, Vol 2018 (2018) |
| ISSN: | 2314-6141 2314-6133 |
| DOI: | 10.1155/2018/7523165 |
| Popis: | The interaction between three flavonoids, i.e., Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) and bovine lactoferrin (BLF) at pH 7.4 was investigated by fluorescence quenching spectra, synchronous fluorescence spectra, and UV-visible absorption spectra. The results indicate the fluorescence of BLF quenched by Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) via static quenching. The main force between QCT and LTL with BLF was van der Waals interactions and hydrogen bonds. Electrostatic interactions played a major role in the binding process of interaction between NGN and BLF. Synchronous fluorescence was used to study the conformational changes of BLF. The values of binding constant (Ka) and number of binding sites (n) at different temperatures (300K, 305K, 310K) were also calculated, respectively. The results of corresponding thermodynamic parameters as well as binding distance between BLF and LTL, QCT, or GNG were obtained. These results implied that Luteolin (LTL), Quercetin (QCT), and Naringenin (NGN) could provide important guides for compound quantity (e.g., medicine dosage) and the design of new compounds (or drugs). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |