Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins
| Autor: | Akjunus Imanbayeva, Valentina Mursaliyeva |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
010405 organic chemistry
Biophysics Plant Science Biology 01 natural sciences Biochemistry 0104 chemical sciences 010404 medicinal & biomolecular chemistry Horticulture Basal shoot Micropropagation Natural source Allochrusa gypsophiloides Turkestan soaproot Saponin-bearing plant Adventitious shoot Micropropagation Biyoloji Biotechnology |
| Zdroj: | Volume: 7, Issue: 1 1-7 International Journal of Secondary Metabolite |
| ISSN: | 2148-6905 |
| Popis: | Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemicto Central Asia, is a valuable saponin-bearing technical and medicinal plant.The purpose of this study was to develop invitro mass propagation for the conservation of endangered species. Nodeexplants were induced to regenerate adventitious shoot buds on Murashige andSkoog medium (MS) supplemented with different concentrations of6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid(NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) wasrecorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one monthcultivation. For elongation, obtained shoot conglomerates were transfered forhormone-free MS. The cultivation of initial explants on MS with kinetin led toa three-fold reduction in the number of shoots characterized by a maximumlength and clearly defined internodes (without the stage of elongation).Micropropagation was achieved by cutting obtained shoots and adventitious shootinduction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required toelongation before rooting on the liquid ½ MS medium contained NAA or IBA. Inboth cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60%, respectively) at 20-day’s incubation. Type of auxin was influenced most onroot quality (thickness, color, branching) than on their frequency and number. Allochrusa gypsophiloides or Turkestan soap root (TSR), which is endemic to Central Asia, is a valuable saponin-bearing technical and medicinal plant. The purpose of this study was to develop in vitro mass propagation for the conservation of endangered species. Node explants were induced to regenerate adventitious shoot buds on Murashige and Skoog medium (MS) supplemented with different concentrations of 6-benzylaminopurine (BAP) and/or kinetin in combination with naphtylacetic acid (NAA). The maximum number of shorter shoots per explant (16.8 ± 3.1) was recorded on MS contained 0.5 mg/L BAP and 0.5 mg/L NAA after one month cultivation. For elongation, obtained shoot conglomerates were transfered for hormone-free MS. The cultivation of initial explants on MS with kinetin led to a three-fold reduction in the number of shoots characterized by a maximum length and clearly defined internodes (without the stage of elongation). Micropropagation was achieved by cutting obtained shoots and adventitious shoot induction. The maximal shoot proliferation (62 ± 6.9) on MS 0.5 mg/L BAP + 1.0 mg/L kinetin + 0.5 mg/L NAA was obtained. Shoots of about 0.5 cm required to elongation before rooting on the liquid ½ MS medium contained NAA or IBA. In both cases, auxin concentration 2.0 mg/L induced maximal rooting (58 % and 60 %, respectively) at 20-day’s incubation. Type of auxin was influenced most on root quality (thickness, color, branching) than on their frequency and number. |
| Databáze: | OpenAIRE |
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