Highly Efficient Abiotic Assay Formats for Methyl Parathion: Molecularly Imprinted Polymer Nanoparticle Assay as an Alternative to Enzyme-Linked Immunosorbent Assay
| Autor: | Sergey A. Piletsky, Todd Cowen, Cem Esen, Joanna Czulak, Elena V. Piletska |
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| Rok vydání: | 2018 |
| Předmět: |
chemistry.chemical_classification
Analyte Chromatography Polymers High-throughput screening 010401 analytical chemistry Molecularly imprinted polymer Enzyme-Linked Immunosorbent Assay Methyl Parathion 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry Molecular Imprinting Enzyme Solid-phase synthesis Tap water chemistry Nanoparticles Magnetic nanoparticles Molecular imprinting |
| Zdroj: | Analytical Chemistry. 91:958-964 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | Enzyme-linked immunosorbent assay (ELISA) is a widely used standard method for sensitive detection of analytes of environmental, clinical, or biotechnological interest. However, ELISA has clear drawbacks related to the use of relatively unstable antibodies and enzyme conjugates and the need for several steps such as washing of nonbound conjugates and addition of dye reagents. Herein, we introduce a new completely abiotic assay where antibodies and enzymes are replaced with fluorescent molecularly imprinted polymer nanoparticles (nanoMIPs) and target-conjugated magnetic nanoparticles, which acted as both reporter probes and binding agents. The components of the molecularly imprinted polymer nanoparticle assay (MINA) are assembled in microtiter plates fitted with magnetic inserts. We have compared the performance of a new magnetic assay with molecularly imprinted polymer (MIP)-based ELISA for the detection of methyl parathion (MP). Both assays have shown high sensitivity toward allowing detection of MP at picomolar concentrations without any cross-reactivity against chlorpyriphos and fenthion. The fully abiotic assays were also proven to detect analyte in real samples such as tap water and milk. Unlike ELISA-based systems, the novel assay required no washing steps or addition of enzyme substrates, making it more user-friendly and suitable for high throughput screening. |
| Databáze: | OpenAIRE |
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