Specific depletion of the house dust mite allergen Der p 1 cereal flour prolamins
| Autor: | R. Sánchez-Monge, M. J. Chamorro, Mónica Pernas, Domingo Barber, J. Carreira, Gabriel Salcedo, C. Arteaga, F. Polo |
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| Rok vydání: | 1996 |
| Předmět: |
Immunology
Flour Wheat flour medicine.disease_cause Acariformes Epitopes Allergen medicine Mite Immunology and Allergy Animals Acari Food science Antigens Dermatophagoides Prolamin Sensitization Glycoproteins Plant Proteins Mites biology Chemistry Pyroglyphidae food and beverages Dust Allergens biology.organism_classification respiratory tract diseases medicine.anatomical_structure Agronomy biology.protein Edible Grain Prolamins |
| Zdroj: | The Journal of allergy and clinical immunology. 97(4) |
| ISSN: | 0091-6749 |
| Popis: | Background: Quantitation of Der 1 and Der 2 in dust samples by specific monoclonal antibodies is a method used increasingly to evaluate mite allergen exposure. The level of Der 1 has been proposed as a risk factor for sensitization. Aim: We report a drastic decrease in the Der 1/Der 2 ratio when dust samples are collected in bakeries. Methods: Wheat flour and purified mites were extracted simultaneously; levels of Der p 1 and Der p 2 and cysteine protease activity were determined by ELISA and inhibition experiments. Results: High titers of Der 2, but only trace amounts of Der p 1, were detected in dust samples collected from bakeries. Both the level and proteolytic activity of Der p 1 appeared greatly decreased when mites and wheat flour were coextracted. Conclusions: Group 1 protein was found to be masked by flour components, resulting in an underestimation of the mite content in bakery dust. This problem was not found for group 2 allergen. |
| Databáze: | OpenAIRE |
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