A novel mycovirus closely related to hypoviruses that infects the plant pathogenic fungus Sclerotinia sclerotiorum
| Autor: | Daohong Jiang, Jiatao Xie, Huiquan Liu, Said A. Ghabrial, Guoqing Li, Xueqiong Xiao, Jiasen Cheng, Yanping Fu |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Untranslated region
DNA Complementary Hypovirulence Sequence analysis Hypoviridae viruses Mycovirus Stem rot Molecular Sequence Data Sequence Homology Genome Viral Rapeseed Open Reading Frames Viral Proteins Ascomycota Virology Cluster Analysis RNA Viruses Cryphonectria Hypovirus Amino Acid Sequence Phylogeny Plant Diseases RNA Double-Stranded biology Base Sequence Sclerotinia sclerotiorum fungi Brassica napus Sequence Analysis DNA biology.organism_classification Satellite-like RNA RNA silencing RNA Viral Sequence Alignment |
| Zdroj: | Virology. 418(1):49-56 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2011.07.008 |
| Popis: | Three dsRNA segments, two similarly sized at 9.5kbp and a third one of approximately 3.6kbp, were extracted from a hypovirulent strain SZ-150 of Sclerotinia sclerotiorum. The complete cDNA sequence of one of the two large dsRNA segment (10398bp, excluding the poly (A) tail) reveals a single ORF that encodes a polyprotein with conserved domains of putative papain-like protease, UDP glucose/sterol glycosyltransferase, RNA-dependent RNA polymerase and viral RNA Helicase. This virus is closely related to Cryphonectria hypovirus (CHV) 3/GH2 and CHV4/SR2 in the family Hypoviridae and designated as Sclerotinia sclerotiorum hypovirus 1 (SsHV1/SZ-150). The satellite-like 3.6kbp dsRNA segment (S-dsRNA) shares high sequence identity with the 5′-UTR of SsHV1/SZ-150. SsHV1/SZ-150 alone is not the primary causal agent for hypovirulence of strain SZ-150 since strains without the S-dsRNA show normal phenotype. This is the first report of a naturally occurring hypovirus that infects a fungus other than Cryphonectria parasitica. |
| Databáze: | OpenAIRE |
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