Gold nanorod-incorporated gelatin-based conductive hydrogels for engineering cardiac tissue constructs
| Autor: | Ryan Sullivan, Ali Navaei, Harpinder Saini, Wayne Christenson, Robert Ros, Mehdi Nikkhah |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
food.ingredient
Materials science Cell Survival Integrin Biomedical Engineering Connexin macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry Gelatin Homogeneous distribution Cell-Matrix Junctions Biomaterials food Tissue engineering Animals Myocytes Cardiac Cell adhesion Molecular Biology Nanotubes Tissue Engineering Tissue Scaffolds biology Electric Conductivity technology industry and agriculture Heart Hydrogels General Medicine 021001 nanoscience & nanotechnology Electric Stimulation Rats 0104 chemical sciences Coupling (electronics) Actin Cytoskeleton Organ Specificity Self-healing hydrogels biology.protein Calcium Gold 0210 nano-technology Biomarkers Biotechnology Biomedical engineering |
| Zdroj: | Acta Biomaterialia. 41:133-146 |
| ISSN: | 1742-7061 |
| DOI: | 10.1016/j.actbio.2016.05.027 |
| Popis: | The development of advanced biomaterials is a crucial step to enhance the efficacy of tissue engineering strategies for treatment of myocardial infarction. Specific characteristics of biomaterials including electrical conductivity, mechanical robustness and structural integrity need to be further enhanced to promote the functionalities of cardiac cells. In this work, we fabricated UV-crosslinkable gold nanorod (GNR)-incorporated gelatin methacrylate (GelMA) hybrid hydrogels with enhanced material and biological properties for cardiac tissue engineering. Embedded GNRs promoted electrical conductivity and mechanical stiffness of the hydrogel matrix. Cardiomyocytes seeded on GelMA-GNR hybrid hydrogels exhibited excellent cell retention, viability, and metabolic activity. The increased cell adhesion resulted in abundance of locally organized F-actin fibers, leading to the formation of an integrated tissue layer on the GNR-embedded hydrogels. Immunostained images of integrin β-1 confirmed improved cell-matrix interaction on the hybrid hydrogels. Notably, homogeneous distribution of cardiac specific markers (sarcomeric α-actinin and connexin 43), were observed on GelMA-GNR hydrogels as a function of GNRs concentration. Furthermore, the GelMA-GNR hybrids supported synchronous tissue-level beating of cardiomyocytes. Similar observations were also noted by, calcium transient assay that demonstrated the rhythmic contraction of the cardiomyocytes on GelMA-GNR hydrogels as compared to pure GelMA. Thus, the findings of this study clearly demonstrated that functional cardiac patches with superior electrical and mechanical properties can be developed using nanoengineered GelMA-GNR hybrid hydrogels. Statement of Significance In this work, we developed gold nanorod (GNR) incorporated gelatin-based hydrogels with suitable electrical conductivity and mechanical stiffness for engineering functional cardiac tissue constructs ( e.g. cardiac patches). The synthesized conductive hybrid hydrogels properly accommodated cardiac cells and subsequently resulted in excellent cell retention, spreading, homogeneous distribution of cardiac specific markers, cell-cell coupling as well as robust synchronized (tissue-level) beating behavior. |
| Databáze: | OpenAIRE |
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