| Autor: |
Dominique C. Stephens, Margaret Mungai, Amber Crabtree, Heather K. Beasley, Edgar Garza-Lopez, Larry Vang, Kit Neikirk, Zer Vue, Neng Vue, Andrea G. Marshall, Kyrin Turner, Jian-qiang Shao, Bishnu Sarker, Sandra Murray, Jennifer A. Gaddy, Jamaine Davis, Steven M. Damo, Antentor O. Hinton |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
bioRxiv |
| Popis: |
Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach.Novel imaging technology is increasing our ability to visualize and analyze cellular organelles and compartments.Tweetable AbstractProper antibody validation of cellular populations within isolated skeletal muscle can lead to better elucidation of skeletal muscle structure and function, and their roles in complex diseases.Graphical AbstractMethod SummaryMyoblasts are isolated from mouse limb muscles. They are plated for immunofluorescence-based validation using confocal microscopy. This method demonstrates the need for reliable antibodies to correctly determine and differentiate between cellular populations within isolated skeletal muscles. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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