Expression of 3 beta-hydroxysteroid dehydrogenase in ovarian and uterine tissue during diestrus and open cervix cystic endometrial hyperplasia-pyometra in the bitch
| Autor: | Nilgün Gültiken, Ayhan Gacar, Gul Fatma Yarim, Murat Yarim, JI Mason |
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| Přispěvatelé: | Ondokuz Mayıs Üniversitesi |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty endocrine system 3-Hydroxysteroid Dehydrogenases animal structures Uterus Ovary Ovarian Medulla Biology Endometrium Canine 03 medical and health sciences Dogs Food Animals Pyometra Internal medicine medicine Animals Dog Diseases Small Animals Cervix Progesterone Equine Cystic Endometrial Hyperplasia Diestrus medicine.disease Endometrial hyperplasia 030104 developmental biology Endocrinology medicine.anatomical_structure Cystic endometrial hyperplasia Endometrial Hyperplasia Animal Science and Zoology Female 3 beta-Hydroxysteroid dehydrogenase |
| Zdroj: | Gultiken, N, Yarim, M, Yarim, G F, Gacar, A & Mason, J I 2016, ' Expression of 3β-hydroxysteroid dehydrogenase in ovarian and uterine tissue during diestrus and open cervix cystic endometrial hyperplasia-pyometra in the bitch ', Theriogenology . https://doi.org/10.1016/j.theriogenology.2016.02.006 |
| DOI: | 10.1016/j.theriogenology.2016.02.006 |
| Popis: | /0000-0002-0636-4214 WOS: 000377843400013 PubMed: 27020880 The purpose of this study was to compare the expression of 3 beta-hydroxystreroid dehydrogenase (3 beta-FISD) in the uterus and ovary of healthy dogs and those with cystic endometrial hyperplasia and/or pyometra complex (CEH-pyometra). Eighteen female dogs were included in the study. Eleven bitches with open cervix CEH-pyometra were included in the CEH-pyometra group and seven diestrus bitches in the control group. For immunostaining a rabbit polyclonal, one raised against recombinant human type 2 (adrenal/gonadal) 3 beta-HSD was used. Progesterone (P4) concentrations were not statistically different between the groups. Strongly stained large interstitial cell groups in the ovarian medulla were observed particularly in CEH-pyometra group although these cells in the control group were weakly or moderately stained and existed singly or paired. The expressions of 3 beta-HSD in luminal epithelium (42.40 +/- 22.40% vs. 18.42 +/- 13.15%, P < 0.05) and glandular epithelium (32.80 +/- 27.05% vs. 2.94 +/- 7.79%, P < 0.01) of endometrium were significantly higher in CEH-pyometra group than those in the control group. The expression of 3 beta-HSD in CL was higher (29.38 +/- 9.58% vs. 22.94 +/- 4.97%) in CEH-pyometra group than that of control group although the differences were not significant (P > 0.05). Similarly, the significant increase in the expression of 3 beta-HSD in ovarian interstitial cells (33.86 +/- 29.44 vs. 1.13 +/- 2.97, P < 0.05) was found in CEH-pyometra group compared to the control group. The study revealed that 3 beta-HSD expression in the endometrium of canine CEH-pyometra was significantly high. (c) 2016 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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