Selection of L cell sublines resistant to (E)-5-(2-bromovinyl)-2'-deoxyuridine
| Autor: | V. Vonka, Fred Rapp, Kimiyasu Shiraki, E. De Clercq |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Biology
medicine.disease_cause Antibodies Viral Virus Replication Antiviral Agents Thymidine Kinase Virus Cell Line chemistry.chemical_compound Mice Virology medicine Animals Simplexvirus Selection Genetic Cell growth Drug Resistance Microbial Molecular biology Deoxyuridine Infectious Diseases Herpes simplex virus chemistry Viral replication Bromodeoxyuridine Thymidine kinase Cell culture |
| Zdroj: | Intervirology. 32(4) |
| ISSN: | 0300-5526 |
| Popis: | A mouse thymidine kinase (TK) deficient L cell subline and L cell sublines biochemically transformed by herpes simplex virus TK were cultured in the presence of increasing concentrations of (E)-5-(2-bromovinyl)-2'-deoxyuridine (BVDU) which inhibited the growth of all sublines, and resistant sublines were isolated. Their growth properties were dependent on the medium condition used for selection. One subline had lost viral TK activity, while another retained viral TK activity with altered sensitivity of TK to BVDU in comparison with that of the original subline. Growth characteristics and TK activity of sublines are discussed. |
| Databáze: | OpenAIRE |
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