Anatomy and activity patterns in a multifunctional motor neuron and its surrounding circuits
| Autor: | Eric A. Bushong, Mária Ashaber, Pegah Kassraian-Fard, Daniel A. Wagenaar, Yusuke Tomina, William B. Kristan, Mark H. Ellisman |
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| Rok vydání: | 2020 |
| Předmět: |
Dorsum
QH301-705.5 Science Leech neural circuit Crawling Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Leeches medicine Animals Biology (General) 030304 developmental biology Motor Neurons 0303 health sciences General Immunology and Microbiology Behavior Animal Staining and Labeling General Neuroscience connectome voltage-sensitive dye imaging General Medicine Segmental ganglion Anatomy Motor neuron Hirudo verbana Ganglion medicine.anatomical_structure Anatomical connectivity serial blockface electron microscopy motor behaviors Connectome Medicine Ganglia Other Research Advance 030217 neurology & neurosurgery Locomotion Neuroscience |
| Zdroj: | eLife eLife, Vol 10 (2021) |
| ISSN: | 2050-084X |
| Popis: | Dorsal Excitor motor neuron DE-3 in the medicinal leech plays three very different dynamical roles in three different behaviors. Without rewiring its anatomical connectivity, how can a motor neuron dynamically switch roles to play appropriate roles in various behaviors? We previously used voltage-sensitive dye imaging to record from DE-3 and most other neurons in the leech segmental ganglion during (fictive) swimming, crawling, and local-bend escape (Tomina and Wagenaar, 2017). Here, we repeated that experiment, then re-imaged the same ganglion using serial blockface electron microscopy and traced all of DE-3’s processes. Further, we traced back the processes of all of DE-3’s presynaptic partners to their respective somata. This allowed us to analyze the relationship between circuit anatomy and the activity patterns it sustains. We found that input synapses important for all of the behaviors were widely distributed over DE-3’s branches, yet that functional clusters were different during (fictive) swimming vs. crawling. |
| Databáze: | OpenAIRE |
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