Accurate in vitro splicing of two pre-mRNA plant introns in a HeLa cell nuclear extract
| Autor: | G. Feix, D. Frendewey, J. W. S. Brown |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
RNA Splicing
Biology General Biochemistry Genetics and Molecular Biology Exon Gene expression RNA Precursors Consensus sequence Humans RNA Messenger Molecular Biology Gene Triticum Cell Nucleus Splice site mutation Base Sequence General Immunology and Microbiology General Neuroscience fungi Intron Nucleic Acid Precursors food and beverages Plants Molecular biology Introns Genes Biochemistry Amylases RNA splicing Precursor mRNA HeLa Cells Research Article |
| Zdroj: | The EMBO Journal. 5:2749-2758 |
| ISSN: | 0261-4189 |
| DOI: | 10.1002/j.1460-2075.1986.tb04563.x |
| Popis: | Two plant introns along with flanking exon sequences have been isolated from an amylase gene of wheat and a legumin gene of pea and cloned behind the phage SP6 promoter. Pre-mRNAs produced by in vitro transcription with SP6 RNA polymerase were tested for their ability to be spliced in a HeLa cell nuclear extract. The plant introns were accurately spliced and the predicted splice junctions were used. Lariat RNAs were observed as both intermediates and final products during the splicing reaction. The branch points were mapped to adenosine residues lying within sequences that showed good homology to the animal branch point consensus. Consensus sequences for the 5' and 3' splice junctions and for putative branch point sequences of plants were derived from an analysis of 168 plant intron sequences. |
| Databáze: | OpenAIRE |
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