Curcumin inhibits MCF-7 cells by modulating the NF-κB signaling pathway
| Autor: | Yan‑Yan Pan, Jing‑Jie Zhao, Xia Xue, Ou Chen, Junli Liu, Ling Liu, Yun Luan, Hong‑Ying Jia |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Articles Cell cycle Biology 03 medical and health sciences chemistry.chemical_compound IκBα 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Oncology MCF-7 chemistry Apoptosis 030220 oncology & carcinogenesis Cancer research Curcumin medicine MTT assay Signal transduction |
| Popis: | The present study investigated the inhibitory effect of curcumin on human breast cancer MCF-7 cells and investigated the potential underlying molecular mechanisms. MCF-7 cells were cultured with curcumin at different concentrations and time points. The effects of curcumin treatment on breast cancer cell proliferation were studied using a MTT assay. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to assess the mRNA and protein expression levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X (Bax), nuclear factor-κ-light-chain-enhancer of activated B cells (NF-κB) and inhibitor of NF-κB-α (IκBα). The proliferation of MCF-7 cells in the group treated with curcumin was markedly decreased compared with the control, with the greatest inhibitory effect at a concentration of 20 µM. The expression of Bax mRNA was increased and Bcl-2 mRNA expression was decreased compared with the control. Additionally, protein expression of NF-κB and IκB was increased. The data indicate that curcumin is able to inhibit breast cancer cell proliferation, possibly by regulating the NF-κB signaling pathway. |
| Databáze: | OpenAIRE |
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