Synergistic enhancement of tendon-to-bone healing via anti-inflammatory and pro-differentiation effects caused by sustained release of Mg2+/curcumin from injectable self-healing hydrogels
| Autor: | Meiguang Xu, Xiaoling Zhou, Zhanhai Yin, Qian Han, Yongping Liang, Meng Li, Baojun Chen, Lang Bai, Jing Zhang, Baolin Guo, Jintao Xiu, Xuezhe Han |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
Curcumin pro-chondrogenesis Anti-Inflammatory Agents Medicine (miscellaneous) 02 engineering and technology Bone healing Bone and Bones Rats Sprague-Dawley Tendons 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Tissue engineering medicine tendon-to-bone healing Animals Rotator cuff Magnesium Pharmacology Toxicology and Pharmaceutics (miscellaneous) 030222 orthopedics Wound Healing Chemistry Stem Cells Cell Differentiation Hydrogels 021001 nanoscience & nanotechnology Chondrogenesis Controlled release rotator cuff anti-inflammation Biomechanical Phenomena Rats medicine.anatomical_structure Delayed-Action Preparations Self-healing hydrogels Drug delivery synergistic effects 0210 nano-technology Biomedical engineering Research Paper |
| Zdroj: | Theranostics |
| ISSN: | 1838-7640 |
| Popis: | Poor healing response after rotator cuff reconstruction is multifactorial, with the inflammatory microenvironment and deficiency of stem cell differentiation factors at the lesion site being most relevant. However, there is a lack of effective tissue engineering strategies that can simultaneously exert anti-inflammatory and pro-differentiation effects to promote rotator cuff healing. Methods: In this study, we synthesized and characterized a novel active drug delivery vector that successfully overcame the challenge of simultaneous high-efficiency loading and controlled release of Mg2+ and curcumin. The anti-inflammatory and pro-differentiation effects of the composite hydrogel were evaluated in vitro and in vivo. Moreover, healing of the rotator cuff tendon-to-bone interface was studied by histology, immunofluorescence, and biomechanical tests. Results: The composite hydrogel exhibited excellent biocompatibility and injectability, good adhesiveness, and rapid self-healing. The released curcumin showed obvious anti-inflammatory and antioxidation effects, which protected stem cells and tendon matrix. Furthermore, released Mg2+ promoted stem cell aggregation and chondrogenesis. Moreover, biomechanical tests and histological results of a rat rotator cuff tear model at 8 weeks after surgery indicated that the composite hydrogel significantly enhanced tendon-to-bone healing. Conclusions: The composite hydrogel mediated sustained in situ release of curcumin and Mg2+ to effectively promote rotator cuff tendon-to-bone healing via anti-inflammatory and pro-differentiation effects. Therefore, this composite hydrogel offers significant promise for rotator cuff repair. |
| Databáze: | OpenAIRE |
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