Cutinase, a lipolytic enzyme with a preformed oxyanion hole
| Autor: | C. Cudrey, H. van Tilbeurgh, Christian Cambillau, Robert Verger, M P Egloff, Aurélie Nicolas, Chrislaine Martinez |
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| Rok vydání: | 1994 |
| Předmět: |
Models
Molecular Cutinase chemistry.chemical_classification Binding Sites biology Protein Conformation Chemistry Stereochemistry Lipolysis Cutinase activity Active site Biochemistry Paraoxon Protein Structure Secondary Recombinant Proteins Kinetics Enzyme Hydrolase Catalytic triad biology.protein Amino Acid Sequence Oxyanion hole Lipase Carboxylic Ester Hydrolases |
| Zdroj: | Biochemistry. 33:83-89 |
| ISSN: | 1520-4995 0006-2960 |
| DOI: | 10.1021/bi00167a011 |
| Popis: | Cutinases, a group of cutin degrading enzymes with molecular masses of around 22-25 kDa (Kolattukudy, 1984), are also able to efficiently hydrolyse triglycerides (De Geus et al., 1989; Lauwereys et al., 1991), but without exhibiting the interfacial activation phenomenom (Sarda et al., 1958). They belong to a class of proteins with a common structural framework, called the alpha/beta hydrolase fold (Martinez et al., 1992; Ollis et al., 1992). We describe herein the structure of cutinase covalently inhibited by diethyl-p-nitrophenyl phosphate (E600) and refined at 1.9-A resolution. Contrary to what has previously been reported with lipases (Brzozowski et al., 1991; Derewenda et al., 1992; Van Tilbeurgh et al., 1993), no significant structural rearrangement was observed here in cutinase upon the inhibitor binding. Moreover, the structure of the active site machinery, consisting of a catalytic triad (S120, H188, D175) and an oxyanion hole (Q121 and S42), was found to be identical to that of the native enzyme, whereas the oxyanion hole of Rhizomucor lipase (Brzozowski et al., 1991; Derewenda et al., 1992), like that of pancreatic lipase (van Tilbeurgh et al., 1993), is formed only upon enzyme-ligand complex formation. The fact that cutinase does not display interfacial activation cannot therefore only be due to the absence of a lid but might also be attributable to the presence of a preformed oxyanion hole. |
| Databáze: | OpenAIRE |
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