A role for LAX2 in regulating xylem development and lateral-vein symmetry in the leaf

Autor: Julieta Virginia Cabello, María E. Otegui, Raquel Lia Chan, Agustín Lucas Arce, Javier E. Moreno, Guillermo S. Moreno-Piovano
Rok vydání: 2017
Předmět:
0106 biological sciences
0301 basic medicine
Auxin influx
AUXIN INFLUX CARRIERS
Otras Ciencias Biológicas
Mutant
Arabidopsis
HaHB4
Plant Science
Real-Time Polymerase Chain Reaction
01 natural sciences
VENATION SYMMETRY
Ciencias Biológicas
03 medical and health sciences
Plant Growth Regulators
Xylem
Auxin
Botany
Oligonucleotide Array Sequence Analysis
chemistry.chemical_classification
Indoleacetic Acids
Auxin homeostasis
biology
Arabidopsis Proteins
VASCULAR PATTERNING
fungi
Wild type
Membrane Transport Proteins
food and beverages
Original Articles
Plants
Genetically Modified
HD-ZIP I
biology.organism_classification
LAX2
Cell biology
Plant Leaves
Complementation
Phenotype
030104 developmental biology
XYLEM ORGANIZATION
chemistry
Soybeans
CIENCIAS NATURALES Y EXACTAS
010606 plant biology & botany
Zdroj: Annals of Botany. 120:577-590
ISSN: 1095-8290
0305-7364
DOI: 10.1093/aob/mcx091
Popis: Background and Aims The symmetry of venation patterning in leaves is highly conserved within a plant species. Auxins are involved in this process and also in xylem vasculature development. Studying transgenic Arabidopsis plants ectopically expressing the sunflower transcription factor HaHB4, it was observed that there was a significant lateral-vein asymmetry in leaves and in xylem formation compared to wild type plants. To unravel the molecular mechanisms behind this phenotype, genes differentially expressed in these plants and related to auxin influx were investigated. Methods Candidate genes responsible for the observed phenotypes were selected using a co-expression analysis. Single and multiple mutants in auxin influx carriers were characterized by morphological, physiological and molecular techniques. The analysis was further complemented by restoring the wild type (WT) phenotype by mutant complementation studies and using transgenic soybean plants ectopically expressing HaHB4. Key Results LAX2, down-regulated in HaHB4 transgenic plants, was bioinformatically chosen as a candidate gene. The quadruple mutant aux1 lax1 lax2 lax3 and the single mutants, except lax1, presented an enhanced asymmetry in venation patterning. Additionally, the xylem vasculature of the lax2 mutant and the HaHB4-expressing plants differed from the WT vasculature, including increased xylem length and number of xylem cell rows. Complementation of the lax2 mutant with the LAX2 gene restored both lateral-vein symmetry and xylem/stem area ratio in the stem, showing that auxin homeostasis is required to achieve normal vascular development. Interestingly, soybean plants ectopically expressing HaHB4 also showed an increased asymmetry in the venation patterning, accompanied by the repression of several GmLAX genes. Conclusions Auxin influx carriers have a significant role in leaf venation pattering in leaves and, in particular, LAX2 is required for normal xylem development, probablt controlling auxin homeostasis. Fil: Moreno Piovano, Guillermo Samuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Moreno, Javier Edgardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Cabello, Julieta Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Arce, Agustín Lucas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina Fil: Otegui, Maria Elena. Universidad de Buenos Aires; Argentina Fil: Chan, Raquel Lia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Databáze: OpenAIRE
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