Transferable vanB2 Tn 5382 -Containing Elements in Fecal Streptococcal Strains from Veal Calves
| Autor: | Kristin H. Dahl, Arnfinn Sundsfjord |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
DNA
Bacterial Fecal streptococci STREPTOCOCCUS LUTETIENSIS Insert (molecular biology) Enterococcus faecalis Microbiology Feces Bacterial Proteins Mechanisms of Resistance Operon Animals Pharmacology (medical) Streptococcus gallolyticus Pharmacology Genetics biology Strain (chemistry) Reverse Transcriptase Polymerase Chain Reaction Superoxide Dismutase Streptococcus biochemical phenomena metabolism and nutrition biology.organism_classification Phenotype Infectious Diseases Genes Bacterial Conjugation Genetic Multigene Family DNA Transposable Elements Cattle Plasmids Enterococcus faecium |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 47:2579-2583 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.47.8.2579-2583.2003 |
| Popis: | Three vancomycin-resistant veal calf fecal streptococci, identified as Streptococcus gallolyticus ( n = 2) and Streptococcus lutetiensis , were shown to harbor vanB2 Tn 5382 -like elements earlier described in enterococci. One S. gallolyticus strain had a 1,495-bp IS 256 -related element inserted in vanS B . The vanB2 Tn 5382 element present in the plasmid-free S. lutetiensis strain was transferable to Enterococcus faecium BM4105-RF, Enterococcus faecalis JH2-2, and its recombination-deficient derivative, UV202. The transfer frequencies were comparable between recipient strains (from 1 × 10 −7 to 7 × 10 −6 ). All transconjugants acquired a vanB -containing chromosomal insert of approximately 100 kb, apparently by site-specific integration. Secondary transconjugants were not observed in intraspecies retransfer experiments. These observations are consistent with a conjugative, selftransmissible, integrative element that might be involved in the interspecies spread of vanB2 resistance determinants. Two JH2-2-derived transconjugants had also gained additional copies of large vanB -containing chromosomal fragments, a process that involves unexplained mechanisms that seems to require functional host cell-dependent recombination mechanisms. |
| Databáze: | OpenAIRE |
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