Analyses of the Kroon Virus Major Capsid Gene and Its Transcript Highlight a Distinct Pattern of Gene Evolution and Splicing among Mimiviruses
| Autor: | Fábio P. Dornas, Rodrigo Araújo Lima Rodrigues, Lorena C. F. Silva, Graziele Pereira Oliveira, Paulo V. M. Boratto, Betânia Paiva Drumond, Juliana R. Cortines, Jônatas Santos Abrahão |
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| Rok vydání: | 2018 |
| Předmět: |
Gene Expression Regulation
Viral 0301 basic medicine Lineage (genetic) Transcription Genetic RNA Splicing Immunology Genome Viral Virus Replication Microbiology Evolution Molecular 03 medical and health sciences Virology Giant Virus Mimiviridae Gene Phylogeny Synteny Genetics Mimivirus Phylogenetic tree biology DNA virus biology.organism_classification 030104 developmental biology Genetic Diversity and Evolution Insect Science RNA Viral Capsid Proteins Water Microbiology |
| Zdroj: | Journal of Virology. 92 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.01782-17 |
| Popis: | The inclusion of Mimiviridae members in the putative monophyletic nucleocytoplasmic large DNA virus (NCLDV) group is based on genomic and phylogenomic patterns. This shows that, along with other viral families, they share a set of genes known as core or “hallmark genes,” including the gene for the major capsid protein (MCP). Although previous studies have suggested that the maturation of mimivirus MCP transcripts is dependent on splicing, there is little information about the processing of this transcript in other mimivirus isolates. Here we report the characterization of a new mimivirus isolate, called Kroon virus (KV) mimivirus. Analysis of the structure, synteny, and phylogenetic relationships of the MCP genes in many mimivirus isolates revealed a remarkable variation at position and types of intronic and exonic regions, even for mimiviruses belonging to the same lineage. In addition, sequencing of KV and Acanthamoeba polyphaga mimivirus (APMV) MCP transcripts has shown that inside the family, even related giant viruses may present different ways to process the MCP mRNA. These results contribute to the understanding of the genetic organization and evolution of the MCP gene in mimiviruses. IMPORTANCE Mimivirus isolates have been obtained by prospecting studies since 2003. Based on genomic and phylogenomic studies of conserved genes, these viruses have been clustered together with members of six other viral families. Although the major capsid protein (MCP) gene is an important member of the so-called “hallmark genes,” there is little information about the processing and structure of this gene in many mimivirus isolates. In this work, we have analyzed the structure, synteny, and phylogenetic relationships of the MCP genes in many mimivirus isolates; these genes showed remarkable variation at position and types of intronic and exonic regions, even for mimiviruses belonging to the same lineage. These results contribute to the understanding of the genetic organization and evolution of the MCP gene in mimiviruses. |
| Databáze: | OpenAIRE |
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