Evidence Suggesting Absence of Mitochondrial DNA Methylation
| Autor: | Odile Fabre, Lars R. Ingerslev, Romain Barrès, Martin Picard, Mie Mechta |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA lcsh:QH426-470 Bisulfite sequencing Biology Deep sequencing 03 medical and health sciences Journal Article Genetics Methylated DNA immunoprecipitation Genetics (clinical) Original Research mtDNA control region DNA methylation epigenetics Methylation Molecular biology whole genome bisulfite sequencing mitochondria lcsh:Genetics 030104 developmental biology Molecular Medicine Illumina Methylation Assay bisulfite sequencing |
| Zdroj: | Frontiers in Genetics Frontiers in Genetics, Vol 8 (2017) Mechta, M, Ingerslev, L R, Fabre, O, Picard, M & Barrès, R 2017, ' Evidence Suggesting Absence of Mitochondrial DNA Methylation ', Frontiers in Genetics, vol. 8, 166 . https://doi.org/10.3389/fgene.2017.00166 |
| ISSN: | 1664-8021 |
| DOI: | 10.3389/fgene.2017.00166 |
| Popis: | Methylation of nuclear genes encoding mitochondrial proteins participates in the regulation of mitochondria function. The existence of cytosine methylation in the mitochondrial genome is debated. To investigate whether mitochondrial DNA (mtDNA) is methylated, we used both targeted- and whole mitochondrial genome bisulfite sequencing in cell lines and muscle tissue from mouse and human origin. While unconverted cytosines were detected in some portion of the mitochondrial genome, their abundance was inversely associated to the sequencing depth, indicating that sequencing analysis can bias the estimation of mtDNA methylation levels. In intact mtDNA, few cytosines remained 100% unconverted. However, removal of supercoiled structures of mtDNA with the restriction enzymeBamHIprior to bisulfite sequencing decreased cytosine unconversion rate to |
| Databáze: | OpenAIRE |
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