Identification, immunoaffinity purification and initial characterization of a novel 71 kD human decidua associated protein by use of specific monoclonal antibodies
| Autor: | Y. Ovadia, P. F. Kraicer, Gideon Fleminger, Reuvit Halperin, Eran Hadas |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Anticorps monoclonal
medicine.drug_class Enzyme-Linked Immunosorbent Assay Pregnancy Proteins Biology Monoclonal antibody Chromatography Affinity Affinity chromatography Pregnancy Decidua Electrochemistry medicine Humans Polyacrylamide gel electrophoresis Decidual tissue chemistry.chemical_classification Immunochemistry Antibodies Monoclonal Cell Biology General Medicine Blood proteins Molecular biology Molecular Weight medicine.anatomical_structure chemistry Electrophoresis Polyacrylamide Gel Female Glycoprotein Developmental Biology |
| Zdroj: | Tissue and Cell. 22:779-784 |
| ISSN: | 0040-8166 |
| DOI: | 10.1016/0040-8166(90)90043-9 |
| Popis: | This study is part of an ongoing attempt to identify and characterize proteins associated with the human decidual tissue. A novel decidual-associated glycoprotein with an apparent molecular weight of 71 kD named hDP71 (human decidual-protein 71), has been identified and purified by immunoaffinity technique using monoclonal antibodies. The monoclonal antibodies recognizing the hDP71 were raised against a partly purified preparation of decidual associated proteins, which was obtained by immunoabsorption of serum proteins from crude decidual extract. Although the hDP71 was copurified with another decidual-associated glycoprotein, the previously described hDP200 (Halperin et al. (1989)), evidence is presented showing that the monoclonal antibodies described above are specific for hDP71. |
| Databáze: | OpenAIRE |
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