Structure and properties of hybrid biopolymer particles fabricated by co-precipitation cross-linking dissolution procedure
| Autor: | Hans Bäumler, Axel Steffen, Kathrin Smuda, Yu Xiong, Radostina Georgieva |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Morphology (linguistics)
Coprecipitation 02 engineering and technology engineering.material 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound Colloid and Surface Chemistry Bovine serum albumin Dissolution biology Chemistry technology industry and agriculture 021001 nanoscience & nanotechnology Microstructure 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Calcium carbonate Chemical engineering biology.protein engineering Particle size Biopolymer 0210 nano-technology |
| Zdroj: | Journal of Colloid and Interface Science. 514:156-164 |
| ISSN: | 0021-9797 |
| DOI: | 10.1016/j.jcis.2017.12.030 |
| Popis: | The Co-precipitation Crosslinking Dissolution technique (CCD-technique) allows a few-steps fabrication of particles composed of different biopolymers and bioactive agents under mild conditions. Morphology and properties of the fabricated biopolymer particles depend on the fabrication conditions, the nature of the biopolymers and additives, but also on the choice of the inorganic templates for co-precipitation. Here, we investigate the influence of an acidic biopolymer, hyaluronic acid (HA), on the formation of particles from bovine hemoglobin and bovine serum albumin applying co-precipitation with CaCO3 and MnCO3. CaCO3 templated biopolymer particles are almost spherical with particle size from 2 to 20 µm and protein entrapment efficiency from 13 to 77%. Presence of HA causes significant structural changes of the particles and decreasing protein entrapment efficiency. In contrast, MnCO3 templated particles exhibit uniform peanut shape and submicron size with remarkably high protein entrapment efficiency of nearly 100%. Addition of HA has no influence on the protein entrapment efficiency or on morphology and size of the particles. These effects can be attributed to the strong interaction of Mn2+ with proteins and much weaker interaction with HA. Therefore, entrapment efficiency, size and structure of biopolymer particles can be optimized by varying the mineral templates and additives. |
| Databáze: | OpenAIRE |
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