Wnt family proteins are secreted and associated with the cell surface
| Autor: | J Papkoff, B D Smolich, J A McMahon, A P McMahon |
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| Rok vydání: | 1993 |
| Předmět: |
Cell signaling
Glycosylation Recombinant Fusion Proteins Cellular differentiation Molecular Sequence Data Wnt1 Protein Wnt2 Protein Biology Transfection Wnt3 Protein Mice Carcinoma Embryonal Proto-Oncogene Proteins Tumor Cells Cultured Animals Pituitary Neoplasms Amino Acid Sequence Molecular Biology Glycoproteins chemistry.chemical_classification Cell Membrane Wnt signaling pathway Proteins LRP6 LRP5 Cell Biology Zebrafish Proteins Molecular biology Extracellular Matrix Neoplasm Proteins Cell biology Wnt Proteins chemistry Multigene Family Signal transduction Glycoprotein Protein Processing Post-Translational Signal Transduction Research Article |
| Zdroj: | Molecular Biology of the Cell. 4:1267-1275 |
| ISSN: | 1939-4586 1059-1524 |
| Popis: | Members of the Wnt gene family are proposed to function in both normal development and differentiation as well as in mammary tumorigenesis. To understand the function of Wnt proteins in these two processes, we present here a biochemical characterization of seven Wnt family members. For these studies, AtT-20 cells, a neuroendocrine cell line previously shown to efficiently process and secrete Wnt-1, was transfected with expression vectors encoding Wnt family members. All of the newly characterized Wnt proteins are glycosylated, secreted proteins that are tightly associated with the cell surface or extracellular matrix. We have also identified native Wnt proteins in retinoic acid-treated P19 embryonal carcinoma cells, and they exhibit the same biochemical characteristics as the recombinant proteins. These data suggest that Wnt family members function in cell to cell signaling in a fashion similar to Wnt-1. |
| Databáze: | OpenAIRE |
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