Association between the expression of chemokine receptors CCR7 and CXCR3, and lymph node metastatic potential in lung adenocarcinoma
| Autor: | Masahide Kuroki, Hirotomo Shibaguchi, Toshihiro Tanaka, Motomu Kuroki, Testushi Kinugasa, Akinori Iwasaki, Takayuki Shirakusa, Jun Yanagisawa, Takehito Kawakami, Shinichi Maekawa |
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| Rok vydání: | 2008 |
| Předmět: |
Cancer Research
Chemokine Pathology medicine.medical_specialty Receptors CCR7 Lung Neoplasms Receptors CXCR3 chemical and pharmacologic phenomena C-C chemokine receptor type 7 Adenocarcinoma Metastasis Immunoenzyme Techniques Chemokine receptor immune system diseases medicine Lymph node stromal cell Tumor Cells Cultured Humans CCR10 Lymph node biology Chemokine CCL21 Chemotaxis hemic and immune systems General Medicine medicine.disease Prognosis medicine.anatomical_structure Oncology Lymphatic Metastasis biology.protein Cancer research Lymph Lymph Nodes |
| Zdroj: | Oncology reports. 19(6) |
| ISSN: | 1021-335X |
| Popis: | Chemokines and their receptors are essential for leukocyte trafficking, and are also involved in cancer metastasis to specific organs. Although the migration of tumor cells into the lymph nodes is an important aspect of cancer, the processes involved are poorly understood. Chemokine receptors CCR7 and CXCR3 have been shown to play an important role in tumor cell migration and lymph node metastasis. Therefore, the assessment of chemokine receptor expression on lung adenocarcinomas may improve the prediction of the spread of this carcinoma to the lymph nodes. In this study, we examined the expression and function of these two chemokine receptors (CCR7 and CXCR3) in lung adenocarcinoma. By using flow cytometry, they were detected in all of the lung adenocarcinoma cell lines examined. In the chemotaxis assays, A549 cells exhibited CCL21-induced migration, which was significantly suppressed by neutralizing anti-CCR7 antibody. The CXCL10-induced migration of A549 cells was also significantly suppressed by neutralizing anti-CXCR3 antibody. In clinical lung adenocarcinoma samples, we found the expression of CCR7 and CXCR3 in 65 and 90% cases, respectively, most of which had lymph node metastasis. Importantly, the expression of CCR7 was significantly associated with lymph node metastasis, although the expression of CXCR3 was not. These results suggest that the activation of CCR7 and CXCR3 with their ligands preferentially stimulates lung adenocarcinoma metastasis to the draining lymph nodes. |
| Databáze: | OpenAIRE |
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