Vacuolization of HeLa cells by a partially purifiedClostridium histolyticumcytotoxin
| Autor: | Tomasz Grzela, Maciej Łazarczyk, Stanislaw Moskalewski, Gayane Martirosian, Justyna Niderla, Ewa Jankowska-Steifer, Jarosław Jóźwiak, Grażyna Korczak–Kowalska, Aldona Komar |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Vacuolar Proton-Translocating ATPases Immunology Apoptosis Vacuole Biology Microbiology HeLa chemistry.chemical_compound Microscopy Electron Transmission Clostridium histolyticum In Situ Nick-End Labeling Humans Immunology and Allergy Propidium iodide Enzyme Inhibitors Cytotoxins Sodium Bafilomycin DNA General Medicine Flow Cytometry biology.organism_classification Molecular biology Infectious Diseases chemistry Biochemistry Vacuolization Neutral Red Vacuoles Clostridium Infections DNA fragmentation Calcium Macrolides HeLa Cells |
| Zdroj: | FEMS Immunology & Medical Microbiology. 46:360-366 |
| ISSN: | 1574-695X 0928-8244 |
| DOI: | 10.1111/j.1574-695x.2006.00041.x |
| Popis: | Clostridium histolyticum vacuolating cytotoxin was partially purified from culture broth using ammonium sulfate precipitation, gel filtration and hydrophobic interaction chromatography. The toxin caused vacuolization of HeLa cells visible under a light microscope after 2 h and distinct after 8 h. Transmission electron microscopy revealed the presence of numerous vacuoles, condensation of the mitochondrial matrix, increased cytoplasm density and increased amounts of heterochromatin. Apoptosis was not detected either by electron microscopy or by an apoptosis/necrosis discrimination assay with fluorescein-labeled annexin V and propidium iodide, or DNA fragmentation assay. Calcium ion influx was detected by flow cytometry after labeling cells with Fluo-4 AM. Vacuolation of HeLa cells by C. histolyticum cytotoxin was inhibited by bafilomycin A1, suggesting involvement of H+ -ATPase in the formation of vacuoles. |
| Databáze: | OpenAIRE |
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