Dietary nucleotides and fecal microbiota in formula-fed infants: a randomized controlled trial
| Autor: | Alun C. Elias-Jones, Julie Lanigan, Sandra Macfarlane, Peter Dudek, Atul Singhal, Kathy Kennedy, George T. Macfarlane, Alan Lucas, Terence Stephenson |
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| Rok vydání: | 2008 |
| Předmět: |
Diarrhea
Male Medicine (miscellaneous) Physiology Gut flora law.invention Feces Randomized controlled trial law Dietary Carbohydrates medicine Birth Weight Humans Bifidobacterium Gastrointestinal tract Nutrition and Dietetics biology Nucleotides Infant biology.organism_classification Dietary Fats Infant Formula Gastrointestinal Tract Breast Feeding Social Class Infant formula Dietary Supplements Immunology Female Dietary Proteins medicine.symptom Energy Intake Breast feeding |
| Zdroj: | The American Journal of Clinical Nutrition. 87:1785-1792 |
| ISSN: | 0002-9165 |
| Popis: | Background Dietary nucleotides are nonprotein nitrogenous compounds that are thought to be important for growth, repair, and differentiation of the gastrointestinal tract. A higher nucleotide intake may also have favorable effects on the fecal microbial composition and incidence of diarrhea in infancy. However, few studies have tested this hypothesis with an experimental study design. Objective We tested the hypothesis that nucleotide supplementation of infant formula has beneficial effects on fecal bacteriology. Design Oligonucleotide probes were used to measure bacterial genus-specific 16S ribosomal RNA in stools of a subset of infants (mean age: 20.4 wk) who were randomly assigned to nucleotide-supplemented (31 mg/L; n = 35) or control formula (n = 37) from birth until age 20 wk or were breastfed (reference group; n = 44). The microbial pattern was assessed as the ratio of Bacteroides-Porphyromonas-Prevotella group (BPP) to Bifidobacterium species. Results The ratio of BPP to Bifidobacterium spp. rRNA in infants randomly assigned to the nucleotide-supplemented formula was lower than in infants receiving the control formula (mean difference: -118%; 95% CI: -203%, -34%; P = 0.007), but it did not differ in infants who were breastfed. The difference between randomized formula-fed groups was independent of potential confounding factors (P = 0.003). Conclusions Our data support the hypothesis that nucleotide supplementation improves the composition of the gut microbiota in formula-fed infants. Because this effect could contribute to previously described benefits of nucleotide supplementation for gastrointestinal tract and immune function, these findings have important implications for optimizing the diet of formula-fed infants. |
| Databáze: | OpenAIRE |
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