MicroRNA Biogenesis is Required for Myc-Induced B cell Lymphoma Development and Survival
| Autor: | Jessica Odvody, Maria Pia Arrate, Rekha Kar, Stephen N. Jones, Tiffaney Vincent, Christine M. Eischen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Ribonuclease III
Cancer Research Lymphoma B-Cell genetic processes Genes myc Biology Article DEAD-box RNA Helicases Mice RNA interference hemic and lymphatic diseases microRNA Endoribonucleases medicine Gene silencing Animals B-cell lymphoma Alleles Mice Knockout fungi food and beverages medicine.disease Genes p53 Phenotype Lymphoma enzymes and coenzymes (carbohydrates) MicroRNAs Cell Transformation Neoplastic Oncology Cancer research biology.protein Haploinsufficiency Gene Deletion Dicer |
| Popis: | Many tumor cells express globally reduced levels of microRNAs (miRNA), suggesting that decreased miRNA expression in premalignant cells contributes to their tumorigenic phenotype. In support of this, Dicer, an RNase III–like enzyme that controls the maturation of miRNA, was recently shown to function as a haploinsufficient tumor suppressor in nonhematopoietic cells. Because the Myc oncoprotein, a critical inducer of B-cell lymphomas, was reported to suppress the expression of multiple miRNAs in lymphoma cells, it was presumed that a deficiency of Dicer and subsequent loss of miRNA maturation would accelerate Myc-induced lymphoma development. We report here that, surprisingly, a haploinsufficiency of Dicer in B cells failed to promote B-cell malignancy or accelerate Myc-induced B-cell lymphomagenesis in mice. Moreover, deletion of Dicer in B cells of CD19-cre+/Eμ-myc mice significantly inhibited lymphomagenesis, and all lymphomas that did arise in these mice lacked functional Cre expression and retained at least one functional Dicer allele. Uncharacteristically, the lymphomas that frequently developed in the CD19-cre+/Dicerfl/fl/Eμ-myc mice were of very early precursor B-cell origin, a stage of B-cell development prior to Cre expression. Therefore, loss of Dicer function was not advantageous for lymphomagenesis, but rather, Dicer ablation was strongly selected against during Myc-induced B-cell lymphoma development. Moreover, deletion of Dicer in established B-cell lymphomas resulted in apoptosis, revealing that Dicer is required for B-cell lymphoma survival. Thus, Dicer does not function as a haploinsufficient tumor suppressor in B cells and is required for B-cell lymphoma development and survival. Cancer Res; 70(14); 6083–92. ©2010 AACR. |
| Databáze: | OpenAIRE |
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