NEW APPROACHES TOWARDS FLUORESCENCE LABELLING OF MESSENGER RNA TRANSCRIPTS

Autor:	Arlette Garnier, Dieter Hüsken, Jan Weiler
Rok vydání:	2001
Předmět:	Messenger RNA Chemistry technology industry and agriculture RNA General Medicine Carbocyanines Biochemistry Photobleaching Fluorescence High resistance Labelling Genetics Nucleic acid Molecular Medicine RNA Messenger Fluorescent Dyes
Zdroj:	Nucleosides, Nucleotides and Nucleic Acids. 20:1181-1185
ISSN:	1532-2335 1525-7770
DOI:	10.1081/ncn-100002515
Popis:	Fluorescence labelling of nucleic acids is being used for a wide range of biological applications. The performance of these techniques is dependent on fluorochrome labels with a high sensitivity and high resistance to photobleaching. Indo-cyanine dyes such as Cy3, Cy5 or Cy7 have been found to fulfill these requirements. This study describes several different RNA labelling techniques allowing for a Cy5 based detection of mRNA transcripts.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ebe3458451f5ffb2013e0d6ef8fe8da1 https://doi.org/10.1081/ncn-100002515 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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