Genotoxicity evaluation of alpha-linolenic acid-diacylglycerol oil

Autor: Aya Hayashi, Hiroshi Honda, Osamu Morita, Yuichi Ito, Naohiro Ikeda, Yurika Fujita
Rok vydání: 2016
Předmět:
TAG
triacylglycerol
Health
Toxicology and Mutagenesis
AF-2
2-(2-furyl)-3-(5-nitro-2-furyl)acrylamide
DMSO
dimethyl sulfoxide
CP
cyclophosphamide
Toxicology
medicine.disease_cause
Alpha-linolenic acid-rich diacylglycerol
Article
Chinese hamster
Ames test
chemistry.chemical_compound
B(a)P
benzo(a)pyrene
0404 agricultural biotechnology
lcsh:RA1190-1270
In vivo
Diacylglycerol oil
medicine
NaN3
sodium azide
MNPCE
micronucleated polychromatic erythrocyte
Alpha-linolenic acid
MMC
mitomycin C
lcsh:Toxicology. Poisons
CLC
Colchicine
Diacylglycerol kinase
biology
urogenital system
alpha-Linolenic acid
04 agricultural and veterinary sciences
biology.organism_classification
040401 food science
2AA
2-aminoanthracene
ICH
International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use
NCE
normochromatic erythrocyte
Biochemistry
chemistry
Micronucleus test
lipids (amino acids
peptides
and proteins)
ALA
alpha-linolenic acid
Diacylglycerol
9AA
9-aminoacridine hydrochloride hydrate
Genotoxicity
Fatty acid composition
DAG
diacylglycerol
LA
linolenic acid
Zdroj: Toxicology Reports
Toxicology Reports, Vol 3, Iss, Pp 716-722 (2016)
ISSN: 2214-7500
DOI: 10.1016/j.toxrep.2016.08.001
Popis: Highlights • We evaluated the genotoxicity of ALA-DAG oil using standard tests. • Bacterial reverse mutation and in vitro/in vivo micronucleus tests were conducted. • No genotoxicity was observed under the testing conditions.
The alpha-linolenic acid (ALA)-diacylglycerol (DAG) oil is an edible oil enriched with DAG (>80%) and ALA (>50%). Although DAG oil, which mainly consists of oleic and linoleic acids has no genotoxic concerns, the fatty acid composition could affect the chemical property of DAG. Therefore, the purpose of this study was to evaluate the genotoxicity of ALA-DAG oil using standard genotoxicity tests in accordance with the OECD guidelines. ALA-DAG oil showed negative results in the bacterial reverse mutation test (Ames test) and in vitro micronucleus test in cultured Chinese hamster lung cells with and without metabolic activation, and in the in vivo bone marrow micronucleus test in mice. Our results did not show any genotoxicity, suggesting that the fatty acid composition had no deleterious effects. We conclude that ALA-DAG oil had no genotoxicity concerns under the testing conditions.
Databáze: OpenAIRE
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