Reference values for interleukin-6 in the amniotic fluid of asymptomatic pregnant women
| Autor: | Ester del Barco, Clara Franco‐Jarava, Mireia Vargas, Nerea Maíz, Silvia Arevalo, Maria Ángeles Sánchez, Maria Teresa Avilés, Carlota Rodó, Manel Mendoza, Maria Goya, Manuel Hernández‐González, Elena Carreras |
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| Přispěvatelé: | Institut Català de la Salut, [Del Barco E, Vargas M, Maíz N, Arevalo S, Sánchez MÁ, Avilés MT, Rodó C, Mendoza M] Unitat de Medicina Materna i Fetal, Servei d’Obstetrícia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. [Franco-Jarava C, Hernández-González M] Servei d’Immunologia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. [Goya M, Carreras E] Unitat de Medicina Materna i Fetal, Servei d’Obstetrícia, Vall d'Hebron Hospital Universitari, Barcelona, Spain. Universitat Autònoma de Barcelona, Bellaterra, Spain. Maternal and Child Health and Development Network (SAMID), Instituto Salud Carlos III, Madrid, Spain, Vall d'Hebron Barcelona Hospital Campus |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: |
Female Urogenital Diseases and Pregnancy Complications::Pregnancy Complications::Fetal Diseases::Chorioamnionitis [DISEASES]
estructuras embrionarias::feto::líquido amniótico [ANATOMÍA] Líquid amniòtic Fluids and Secretions::Amniotic Fluid [ANATOMY] Persons::Women::Pregnant Women [NAMED GROUPS] Infecció Obstetrics and Gynecology Embaràs - Complicacions General Medicine enfermedades de los genitales femeninos y complicaciones del embarazo::complicaciones del embarazo::enfermedades fetales::corioamnionitis [ENFERMEDADES] personas::mujeres::mujeres embarazadas [DENOMINACIONES DE GRUPOS] |
| Zdroj: | Scientia |
| Popis: | Amniocentesis; Interleukin-6; Intra-amniotic inflammation Amniocentesi; Interleucina-6; Inflamació intraamniòtica Amniocentesis; Interleucina-6; Inflamación intraamniótica Introduction Nowadays, proinflammatory factors are considered to play an important role in the pathophysiology of threatened preterm labor or chorioamnionitis. The aim of this study was to establish the normal reference range for interleukin-6 (IL-6) levels in the amniotic fluid and to identify factors which may alter this value. Material and methods Prospective study in a tertiary-level center including asymptomatic pregnant women undergoing amniocentesis for genetic studies from October 2016 to September 2019. IL-6 measurements in amniotic fluid were performed using a fluorescence immunoassay with microfluidic technology (ELLA Proteinsimple, Bio Techne). Maternal history and pregnancy data were also recorded. Results This study included 140 pregnant women. Of those, women who underwent termination of pregnancy were excluded. Therefore, a total of 98 pregnancies were included in the final statistical analysis. The mean gestational age was 21.86 weeks (range: 15–38.7) at the time of amniocentesis, and 38.6 weeks (range: 30.9–41.4) at delivery. No cases of chorioamnionitis were reported. The log10 IL-6 values follow a normal distribution (W = 0.990, p = 0.692). The median, and the 5th, 10th, 90th, and 95th percentiles for IL-6 levels were 573, 105, 130, 1645, and 2260 pg/mL, respectively. The log10 IL-6 values were not affected by gestational age (p = 0.395), maternal age (p = 0.376), body mass index (p = 0.551), ethnicity (p = 0.467), smoking status (p = 0.933), parity (p = 0.557), method of conception (p = 0.322), or diabetes mellitus (p = 0.381). Conclusions The log10 IL-6 values follow a normal distribution. IL-6 values are independent of gestational age, maternal age, body mass index, ethnicity, smoking status, parity and method of conception. Our study provides a normal reference range for IL-6 levels in the amniotic fluid that can be used in future studies. We also observed that normal IL-6 values were higher in the amniotic fluid than in serum. Some of the authors are members of the European Reference Network on Rare Congenital Malformations and Rare Intellectual Disability ERN-ITHACA (EU Framework Partnership Agreement ID: 3HP-HP-FPA ERN-01-2016/739516). |
| Databáze: | OpenAIRE |
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