Protective effects of Cassia tora leaves in experimental cataract by modulating intracellular communication, membrane co-transporters, energy metabolism and the ubiquitin-proteasome pathway

Autor: Sreelakshmi, Annie Abraham
Rok vydání: 2017
Předmět:
0301 basic medicine
Intracellular Fluid
Cassia tora
genetic structures
Pharmaceutical Science
Cell Communication
Pharmacology
medicine.disease_cause
functional food
Rats
Sprague-Dawley
Subcutaneous injection
0302 clinical medicine
Drug Discovery
biology
Symporters
Calpain
General Medicine
Treatment Outcome
Biochemistry
selenite cataract
Molecular Medicine
blindness
Signal Transduction
Proteasome Endopeptidase Complex
Cassia
Protective Agents
stat
Cataract
03 medical and health sciences
Functional food
medicine
Animals
Blindness
Dose-Response Relationship
Drug
business.industry
Plant Extracts
Ubiquitin
lcsh:RM1-950
Cell Membrane
medicine.disease
eye diseases
Rats
Plant Leaves
Oxidative Stress
030104 developmental biology
lcsh:Therapeutics. Pharmacology
Complementary and alternative medicine
Proteasome
Dietary Supplements
030221 ophthalmology & optometry
biology.protein
business
Energy Metabolism
Oxidative stress
Zdroj: Pharmaceutical Biology, Vol 55, Iss 1, Pp 1274-1282 (2017)
ISSN: 1744-5116
Popis: Context: Cataract is the clouding of eye lens which causes impairment in vision and accounts for the leading factor of global blindness. Functional food-based prevention of cataract finds application in vision research because of its availability and easy access to all classes of the society. Cassia tora Linn. (Caesalpinaceae) is an edible plant mentioned in the traditional systems of medicine for whole body health, especially to the eyes. Objective: The present study evaluates the potential of ethyl acetate fraction of Cassia tora leaves (ECT) on experimental cataract. Materials and methods: Cataract was induced by a single subcutaneous injection of sodium selenite (4 μg/g body weight) on 10th day. ECT was supplemented orally from 8th day up to 12th day at a concentration of 5 μg/g body weight and marker parameters were evaluated after 30 days. Results: The production of MPO and the activation of calpain were reduced 52.17% and 36.67% by ECT in lens tissue, respectively. It modulated the energy status by significantly increasing the activity of CCO 1 (55.56%) and ATP production (41.88%). ECT maintained the ionic balance in the lens by reducing the level of sodium (50%) and increasing the level of potassium (42.5%). It also reduced cell junction modifications and preserved a functional ubiquitin-proteasome pathway. Discussion and conclusion: The results reinforce the growing attention on wild plant food resources for preventive protection against cataract. The data suggest the value of Cassia tora leaves as a functional food for ameliorating cataract pathology.
Databáze: OpenAIRE
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