LIPH expression in skin and hair follicles of normal coat and Rex rabbits
| Autor: | Stephan Bouet, Mathieu Diribarne, Xavier Mata, Séverine Deretz, Fabienne Reine, Laurent Schibler, Gérard Auvinet, Edmond-Paul Cribiu, Jérôme Chapuis, Julie Rivière, Gérard Guérin, Daniel Allain, Renaud Fleurot, Anne Vaiman |
|---|---|
| Přispěvatelé: | Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Génétique Expérimentale en Productions Animales (GEPA), Station d'Amélioration Génétique des Animaux (SAGA), laboratoire de Virologie Immunologie Moléculaire, INRA, Jouy, AgroParisTech-Institut National de la Recherche Agronomique (INRA), Guérin, Gerard |
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
Anatomy and Physiology Mutant lcsh:Medicine MESH: Rabbits MESH: Cricetinae Outer root sheath Inner root sheath MESH: Lipase MESH: Genotype MESH: Mutant Proteins 0302 clinical medicine MESH: Cricetulus Mutant protein Cricetinae MESH: Reverse Transcriptase Polymerase Chain Reaction Gene expression MESH: Gene Expression Regulation Developmental mutation rex rabbit liph gene skin hair follicle MESH: Animals lcsh:Science MESH: Phospholipases A1 In Situ Hybridization Sequence Deletion Animal Management 0303 health sciences Multidisciplinary integumentary system Reverse Transcriptase Polymerase Chain Reaction MESH: Gene Expression Regulation Enzymologic Gene Expression Regulation Developmental Agriculture Genomics MESH: Sequence Deletion Immunohistochemistry medicine.anatomical_structure Phenotype 030220 oncology & carcinogenesis Medicine Female MESH: Hair Follicle Rabbits MESH: Hair Research Article MESH: Mutation Genotype CHO Cells Dermatology Biology Transfection MESH: Phenotype Gene Expression Regulation Enzymologic Molecular Genetics 03 medical and health sciences Cricetulus MESH: In Situ Hybridization MESH: Skin MESH: CHO Cells [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] medicine Genetics Animals 030304 developmental biology Messenger RNA MESH: Transfection lcsh:R Wild type MESH: Immunohistochemistry Lipase Hair follicle Molecular biology Phospholipases A1 MESH: Male Mutant Proteins lcsh:Q MESH: Female Hair |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2012, 7 (1), pp.e30073. ⟨10.1371/journal.pone.0030073⟩ PLoS ONE, Vol 7, Iss 1, p e30073 (2012) Plos One 1 (7, online), Non paginé. (2012) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0030073⟩ |
| Popis: | Chantier qualité spécifique "Auteurs Externes" département de Génétique animale : uniquement liaison auteur au référentiel HR-Access; International audience; Natural mutations in the LIPH gene were shown to be responsible for hair growth defects in humans and for the rex short hair phenotype in rabbits. In this species, we identified a single nucleotide deletion in LIPH (1362delA) introducing a stop codon in the C-terminal region of the protein. We investigated the expression of LIPH between normal coat and rex rabbits during critical fetal stages of hair follicle genesis, in adults and during hair follicle cycles. Transcripts were three times less expressed in both fetal and adult stages of the rex rabbits than in normal rabbits. In addition, the hair growth cycle phases affected the regulation of the transcription level in the normal and mutant phenotypes differently. LIPH mRNA and protein levels were higher in the outer root sheath (ORS) than in the inner root sheath (IRS), with a very weak signal in the IRS of rex rabbits. In vitro transfection shows that the mutant protein has a reduced lipase activity compared to the wild type form. Our results contribute to the characterization of the LIPH mode of action and confirm the crucial role of LIPH in hair production. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|