A Porcine Model of Staphylococcus epidermidis Catheter-Associated Infection
| Autor: | J. W. Costerton, K P McDermid, N D Boyd, Merle E. Olson, Douglas W. Morck, A. E. Khoury, M. K. Dasgupta |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Pathology
medicine.medical_specialty Micrococcaceae Swine Staphylococcus Blood Urea Nitrogen Serology Microbiology Catheters Indwelling Immune system Antigen Staphylococcus epidermidis medicine Animals Immunology and Allergy Antiserum biology business.industry Staphylococcal Infections biology.organism_classification Catheter Infectious Diseases Creatinine Immunoglobulin G Antibody Formation Female business Peritoneal Dialysis Bacteria |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1537-6613 0022-1899 |
| DOI: | 10.1093/infdis/168.4.897 |
| Popis: | The suitability of using catheterized, partially nephrectomized (uremic) pigs to study catheter-associated infection in peritoneal dialysis was investigated. In some pigs, an inoculum of 10(9) cfu of Staphylococcus epidermidis ATCC 35984 was deposited around the catheter exit site and the organism was allowed to colonize over 21 days. The strain was recoverable from tissues and catheter samples at various locations along the catheter tract from inoculated pigs at postmortem examination. Uninoculated control catheters were colonized to a significantly lesser degree and by various other staphylococcal species. Immune response by inoculated pigs toward catheter-associated bacteria was indicated by a significant increase in serum antistaphylococcal IgG concentration and an increased percentage of peripheral polymorphonuclear leukocytes. Uremia had no significant effect on serologic response. Immunoblotting against S. epidermidis lysostaphin-extracted proteins showed that although incubation with inoculated group antisera produced more intense banding and reacted to a wider range of protein than did antisera from uninoculated controls, common antigenic proteins among the groups were found. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|