Detection of Foot-and-Mouth Disease Virus in the Absence of Clinical Disease in Cattle and Buffalo in South East Asia
| Autor: | Chattouphone Keokhamphet, Carolyn Hamill, Ashish Sutar, Rudolfo Bueno, Sengxay Phonthasy, Viliddeth Souriya, Wendi D. Roe, Syseng Khounsy, Richard Spence, F Castillo-Alcala, Ronel Abila, Bolortuya Purevsuren, Myo Thiha, Khin Ohnmar Lwin, Andrew McFadden, Anna B. Ludi, Jonathan Arzt, Valerie Mioulet, Kelly Buckle, Blesilda Verin, Emilie Vallée, Mary van Andel, Htun Htun Win |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Veterinary medicine medicine.medical_specialty 040301 veterinary sciences abattoirs Mucous membrane of nose Virus 0403 veterinary science 03 medical and health sciences buffaloes SF600-1100 Epidemiology medicine Subclinical infection General Veterinary biology Foot-and-mouth disease foot-and-mouth disease virus Outbreak 04 agricultural and veterinary sciences Brief Research Report biology.organism_classification medicine.disease reverse transcriptase polymerase chain reaction 030104 developmental biology Nasal Swab cattle foot-and-mouth disease surveillance Veterinary Science Foot-and-mouth disease virus |
| Zdroj: | Frontiers in Veterinary Science Frontiers in Veterinary Science, Vol 8 (2021) |
| ISSN: | 2297-1769 |
| Popis: | Foot-and-mouth disease virus (FMDV) is widespread throughout much of the world, including parts of South East Asia. Surveillance is often limited in endemic areas, relying predominantly on passive outbreak reporting. As part of the World Organisation for Animal Health (OIE)'s South East Asia and China Foot-and-Mouth Disease Project (SEACFMD), field sampling was performed to help understand evidence of widespread virus exposure observed in previous studies. Serum and dry mucosal swabs were collected to evaluate the presence of FMDV RNA on the nasal, oral, and dorsal nasopharyngeal mucosal surfaces of 262 healthy cattle (n = 84 in Laos; n = 125 in Myanmar) and buffalo (n = 48 in Laos; n = 5 in Myanmar) immediately following slaughter in three slaughterhouses. Swabs and serum were tested by the OIE/FAO World Reference Laboratory for foot-and-mouth disease (WRLFMD) using pan-serotypic real-time reverse transcription-PCR (rRT-PCR) and serum was evaluated using the FMD PrioCHECK non-structural protein (NSP) ELISA. In total, 7.3% of animals had detectable FMDV RNA in one or more of the three sites including 5.3% of nasopharyngeal swabs, 2.3% of oral swabs, and 1.5% of nasal swabs. No FMDV RNA was detected in serum. Overall, 37.8% of animals were positive for NSP antibodies, indicating likely past natural exposure to FMDV. Results were comparable for Laos and Myanmar, and for both cattle and buffalo, and were not significantly different between age groups. Detectable FMDV RNA present on the oral and nasal mucosa of clinically-healthy large ruminants in Laos and Myanmar demonstrates the importance of sampling asymptomatic animals as part of surveillance, and may indicate that subclinical infection plays a role in the epidemiology of FMD in these countries. |
| Databáze: | OpenAIRE |
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