Down-regulation of the class I HLA heterodimer and beta2-microglobulin on the surface of cells infected with cytomegalovirus
| Autor: | Penelope D. Barnes, Jane E. Grundy |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Human cytomegalovirus
Cell Membrane Permeability Macromolecular Substances T cell Cell Cytomegalovirus Down-Regulation Fluorescent Antibody Technique Human leukocyte antigen Biology medicine.disease_cause Virus Herpesviridae HLA Antigens Virology medicine Humans Cytotoxic T cell Cells Cultured Beta-2 microglobulin Histocompatibility Antigens Class I Biological Transport Fibroblasts medicine.disease Cell Compartmentation medicine.anatomical_structure Antigens Surface beta 2-Microglobulin |
| Zdroj: | Journal of General Virology. 73:2395-2403 |
| ISSN: | 1465-2099 0022-1317 |
| DOI: | 10.1099/0022-1317-73-9-2395 |
| Popis: | Cytotoxic T cell recognition of virus-infected cells requires the presentation of viral peptides by class I HLA molecules on the cell surface. We report here that cytomegalovirus (CMV) infection of human fibroblasts results in a progressive decrease in the cell surface expression of class I HLA and beta 2-microglobulin (beta 2m) such that in the late stages of infection the majority of infected cells have no detectable surface class I HLA. Coincident with decreased surface expression of class I HLA was an increase in his cytoplasmic expression. Confocal scanning laser microscopic analysis demonstrated that class I HLA and beta 2m accumulate in a perinuclear compartment inside the CMV-infected cell. Our data thus support the concept that CMV infection induces altered transport of class I HLA to the cell surface. We suggest that the virus has evolved this mechanism as a strategy to avoid T cell recognition of infected cells. |
| Databáze: | OpenAIRE |
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