Human papillomavirus DNA in multicentric vulvar intraepithelial neoplasia
| Autor: | F. J.W. Ten Kate, N. van der Vange, M. Van Beurden, S. P. Tjong-A-Hung, J. Ter Schegget, F. B. Lammes, A. J. M. De Craen |
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| Přispěvatelé: | Other departments |
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Vaginal Neoplasms Uterine Cervical Neoplasms Biology Cervical intraepithelial neoplasia Pathology and Forensic Medicine Vulva law.invention law medicine Humans Cervix Papillomaviridae Polymerase chain reaction Vaginal intraepithelial neoplasia Vulvar Neoplasms Carcinoma in situ Obstetrics and Gynecology virus diseases Vulvar intraepithelial neoplasia medicine.disease Koilocyte female genital diseases and pregnancy complications medicine.anatomical_structure DNA Viral Female |
| Zdroj: | International journal of gynecological pathology, 17(1), 12-16. Lippincott Williams and Wilkins |
| ISSN: | 0277-1691 |
| DOI: | 10.1097/00004347-199801000-00003 |
| Popis: | Human papillomavirus (HPV) DNA in vulvar intraepithelial neoplasia (VIN) is associated with multifocality of VIN III and with multicentricity of other neoplastic squamous lesions in the cervix and vagina. The aim of this study was to establish the prevalence and type of HPV DNA in the lesions of vaginal intraepithelial neoplasia (VaIN) and cervical intraepithelial neoplasia (CIN) in patients with VIN III using the polymerase chain reaction (PCR). HPV DNA detection and histologic analysis were performed on alternating sections of paraffin-embedded biopsies of concomitant CIN and VaIN in 27 patients with VIN III. PCR was performed with consensus primers and HPV typing was performed by direct sequencing of the PCR amplimers. HPV DNA was detected in all VIN III lesions (93% contained HPV-16 DNA); in 96% of the CIN lesions (73% contained HPV-16 DNA); and in all VaIN lesions (75% contained HPV-16 DNA). The HPV type was not the same in 22% of the different lesions of VIN, CIN, and VaIN, even if the biopsies were taken at the same time. |
| Databáze: | OpenAIRE |
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