Quantification of Antibiotic in Biofilm-Inhibiting Multilayers by 7.87 eV Laser Desorption Postionization MS Imaging
| Autor: | Luke Hanley, Praneeth D. Edirisinghe, Artem Akhmetov, M T Melvin Blaze, Berdan Aydin, Gulsah Uygur |
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| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
biology
Lasers Analytical chemistry Biofilm Microbial Sensitivity Tests biology.organism_classification Mass spectrometry Small molecule Enterococcus faecalis Polyelectrolyte Article Mass Spectrometry Analytical Chemistry Anti-Bacterial Agents Molecular Imaging Chitosan chemistry.chemical_compound Adsorption chemistry Desorption Biofilms Acetamides Ampicillin |
| Popis: | The potential of laser desorption postionization mass spectrometry (LDPI-MS) imaging for small molecule quantification is demonstrated here. The N-methylpiperazine acetamide (MPA) of ampicillin was adsorbed into polyelectrolyte multilayer surface coatings composed of chitosan and alginate, both high molecular weight biopolymers. These MPA-ampicillin spiked multilayers were then shown to inhibit the growth of Enterococcus faecalis biofilms that play a role in early stage infection of implanted medical devices. Finally, LDPI-MS imaging using 7.87 eV single-photon ionization was found to detect MPA-ampicillin within the multilayers before and after biofilm growth with limits of quantification and detection of 0.6 and 0.3 nmol, respectively. The capabilities of LDPI-MS imaging for small molecule quantification are compared to those of MALDI-MS. Furthermore, these results indicate that 7.87 eV LDPI-MS imaging should be applicable to quantification of a range of small molecular species on a variety of complex organic and biological surfaces. Finally, while MS imaging for quantification was demonstrated here using LDPI, it is a generally useful strategy that can be applied to other methods. |
| Databáze: | OpenAIRE |
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