Glucose deprivation-induced cytotoxicity in drug resistant human breast carcinoma MCF-7/ADR cells: role of c-myc and bcl-2 in apoptotic cell death
| Autor: | Christine M. Berns, Peter M. Corry, Sandra Galoforo, Yong J. Lee, William P. Tong, Hyeong Reh Choi Kim |
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| Rok vydání: | 1997 |
| Předmět: |
Regulation of gene expression
Programmed cell death Cell Survival Genes myc Apoptosis Breast Neoplasms Cell Biology Transfection Biology medicine.disease Molecular biology Drug Resistance Multiple Genes bcl-2 Gene Expression Regulation Neoplastic Glucose Drug Resistance Neoplasm Gene expression Carcinoma medicine Tumor Cells Cultured Humans Northern blot Cytotoxicity |
| Zdroj: | Journal of cell science. 110 |
| ISSN: | 0021-9533 |
| Popis: | We investigated the effect of glucose deprivation treatment on clonogenicity in multidrug-resistant human breast carcinoma MCF-7/ADR cells. Survival of MCF-7/ADR cells decreased exponentially up to 8 hours of incubation in the glucose-free medium. The surviving fraction of these cells for 8 hours of glucose-deprivation treatment was 1.5 × 10(−3). Photomicrographs and gel electrophoresis data suggest that glucose deprivation-induced cell death is associated with apoptosis. Data from western and northern blots showed an induction of c-myc gene expression during treatment with glucose-free medium in MCF-7/ADR cells. MCF-7/ADR cells transfected with c-myc antisense oligodeoxynucleotides became resistant to glucose deprivation-induced apoptosis. Overexpression of bcl-2 gene protected MCF-7/ADR cells from this apoptotic cell death. Taken together, these results indicate that c-myc expression is a necessary component of glucose-free medium induced apoptosis and bcl-2 prevents apoptotic death induced by c-myc. |
| Databáze: | OpenAIRE |
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