Rapid separation and purification of two C25 steroids with bicyclic [4.4.1] A/B rings from the marine fungus Aspergillus sp. LS116 by high-speed counter-current chromatography in stepwise elution mode
| Autor: | Bin Zhang, Peng Xu, Lijian Ding, Xiaoping He, Zhende Dou |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Aspergillus
Chromatography biology Bicyclic molecule 010405 organic chemistry medicine.medical_treatment Organic Chemistry Ethyl acetate Plant Science biology.organism_classification 01 natural sciences Biochemistry 0104 chemical sciences Analytical Chemistry Steroid 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Countercurrent chromatography chemistry medicine Stepwise elution Petroleum ether Methanol |
| DOI: | 10.6084/m9.figshare.14034306.v1 |
| Popis: | Two C25 steroids with bicyclic [4.4.1] A/B rings were successfully separated from the marine fungus Aspergillus sp. LS116 by a two-step high-speed counter current chromatography (HSCCC). Petroleum ether/ethyl acetate/methanol/water (5.5:11:5:7, v/v) and petroleum ether/ethyl acetate/methanol/water (5:6:5:7, v/v) were selected as two optimum two-phase systems to purify two C25 steroids, neocyclocitrinol B (1) and threo-23-O-methylneocyclocitrinol (2). The purity of two compounds was over 94%. Their structures were determined by comprehensive spectroscopic techniques. This is the first report about rapid separation and identification of C25 steroids with bicyclic [4.4.1] A/B rings by HSCCC. |
| Databáze: | OpenAIRE |
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