Morphology and histology of paryphasmata and hemibaculum of Varanus salvator based on sexual maturity
| Autor: | Benjamin Christoffel Tehupuring, Boedi Setiawan, Imam Mustofa, Suzanita Utama, Chairul Anwar, Djoko Legowo, Lita Rakhma Yustinasari, Eka Pramyrtha Hestianah, Regita Permata Sari, Alif Yahya Al-Ma'ruf, Maslichah Mafruchati |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Loose connective tissue
Dense connective tissue snout-vent length General Veterinary Ossification Hyaline cartilage chondrocytes Histology Lizards Anatomy Biology sexual maturity medicine.anatomical_structure QL1-991 Hemipenis medicine Sexual maturity Animals Humans medicine.symptom glans hemipenis Zoology Hyaline Original Research |
| Zdroj: | Open Veterinary Journal, Vol 11, Iss 2, Pp 330-336 (2021) Open Veterinary Journal |
| ISSN: | 2218-6050 |
| Popis: | Background: Varanus salvator is one of the reptiles being hunted by human beings for several purposes, including traditional medicine. The studies about reproductive biology aspects were limited. Aim: This study aimed to determine the morphology, histology, and histometry of V. salvator paryphasmata and hemibaculum based on Snout-Vent Length (SVL) as an indicator of sexual maturity. Methods: This study examined 18 pairs of hemipenis of V. salvator with SVL more and less than 40 cm in equal number. Paryphasmata and hemibaculum parts were observed visually and micro-sliced, then stained with Hematoxylin-Eosin (HE). The histological observation was conducted under a 40×, 100×, and 400× magnification of a light microscope. The histometry of the paryphasmata was examined using 13 Megapixels Coolpad and OptiLab Plus for microscopic pictures. The chondrocyte cell area was measured using the Optilab Plus and Image Raster three applications. Results: The sizes of glans of hemipenis, paryphasmata, and hemibaculum increased according to the increasing of SVL. The average paryphasmata row number, epidermis, and loose connective tissue thickness were not significantly different (p > 0.05). However, dense connective tissue was thicker (p < 0.05), which corresponds to SVL. Hemibaculum was composed of fibrous and hyaline cartilage characterized by chondrocyte cells. The SVL also affects (p < 0.05) the ossification of hyaline in hemipenis, while the chondrocyte cell area followed the equation −1.87E7 + 7.09E5* SVL. Conclusion: The SVL size of V. salvator affects the paryphasmata, hemibaculum, thickness of dense connective tissue of paryphasmata, and the area of chondrocyte cells. |
| Databáze: | OpenAIRE |
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