Subclinical endometritis in dairy cattle is associated with distinct mRNA expression patterns in blood and endometrium

Autor: Doulaye Dembélé, Iain Martin Sheldon, Mariam Raliou, M. Heppelmann, Sophie Mockly, Hans-Joachim Schuberth, Peter Zieger, Julie Aubert, Isabelle Dieuzy-Labaye, Tristan Mary-Huard, Dominique Rocha, Francois Piumi, Anna Düvel, David Smith, Philippe Bolifraud, Olivier Sandra
Přispěvatelé: Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université Paris Saclay (COMUE), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), University of Veterinary Medicine Hanover, Mathématiques et Informatique Appliquées (MIA-Paris), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Zoetis [Zaventem], Zoetis [Berlin], Institute of Infection, Immunology and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Swansea University, ERA-NET EMIDA (Coordination of European Research on Emerging and Major Infectious Diseases of Livestock), 2010, Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), ProdInra, Archive Ouverte, École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2019
Předmět:
0301 basic medicine
Microarray
Physiology
Biopsy
subclinical endometritis
cow
Gene Expression
Endometrium
Transcriptome
White Blood Cells
Animal Cells
Leukocytes
Medicine and Health Sciences
dairy cows
postpartum
Subclinical infection
2. Zero hunger
Mammals
Multidisciplinary
medicine.diagnostic_test
microarray
circulating white blood cells
endometrium
Eukaryota
04 agricultural and veterinary sciences
Ruminants
Body Fluids
Dairying
medicine.anatomical_structure
Blood
Veterinary Diseases
expression de l'arn m
vache
Vertebrates
endomètre
Medicine
Veterinary medicine and animal Health
Female
Endometritis
Anatomy
Cellular Types
Research Article
Science
Immune Cells
Immunology
Cattle Diseases
Surgical and Invasive Medical Procedures
Estrous Cycle
Biology
Andrology
03 medical and health sciences
Bovines
medicine
Genetics
Animals
RNA
Messenger
Estrous cycle
[SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
Blood Cells
Uterus
[SDV.BA.MVSA] Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health
0402 animal and dairy science
Reproductive System
Organisms
Biology and Life Sciences
Cell Biology
medicine.disease
040201 dairy & animal science
030104 developmental biology
Médecine vétérinaire et santé animal
Amniotes
Cattle
Veterinary Science
Physiological Processes
Postpartum period
Endometrial biopsy
Zdroj: PLoS ONE
Plos One 8 (14), . (2019)
PLoS ONE, Vol 14, Iss 8, p e0220244 (2019)
PLoS ONE, Public Library of Science, 2019, 14 (8), ⟨10.1371/journal.pone.0220244⟩
PLoS ONE, 2019, 14 (8), ⟨10.1371/journal.pone.0220244⟩
ISSN: 1932-6203
Popis: Cattle with subclinical endometritis (SCE) are sub-fertile and diagnosing subclinical uterine disease remains a challenge. The hypothesis for this study was that endometrial inflammation is reflected in mRNA expression patterns of peripheral blood leucocytes. Transcriptome profiles were evaluated in healthy cows and in cows with SCE using circulating white blood cells (WBC) and endometrial biopsy samples collected from the same animals at 45-55 days postpartum. Bioinformatic analyses of microarray-based transcriptional data identified gene profiles associated with distinct biological functions in circulating WBC and endometrium. In circulating WBC, SCE promotes a pro-inflammatory environment, whereas functions related to tissue remodeling are also affected in the endometrium. Nineteen differentially expressed genes associated with SCE were common to both circulating WBC and the endometrium. Among these genes, transcript abundance of immune factors C3, C2, LTF, PF4 and TRAPPC13 were up-regulated in SCE cows at 45-55 days postpartum. Moreover, mRNA expression of C3, CXCL8, LTF, TLR2 and TRAPPC13 was temporally regulated during the postpartum period in circulating WBC of healthy cows compared with SCE cows. This observation might indicate an advantageous modulation of the immune system in healthy animals. The transcript abundance of these genes represents a potential source of indicators for postpartum uterine health.
Databáze: OpenAIRE
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