CD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis
| Autor: | Momin Khan, Varinder K. Aggarwal, Ryan O. Emerson, Zuzanna Z. Moleda, Adriaan J. Minnaard, Martine Gilleron, D. Branch Moody, Charlotte A. James, Peter Reinink, Josephine F. Reijneveld, Dale I. Godfrey, Thomas J. Scriba, Michael N. T. Souter, Eleonora Diamanti, Krystle K. Q. Yu, Chetan Seshadri, Ildiko Van Rhijn, Stefanie Lenz, Daniel G. Pellicci, Vijayendar R. Yedulla, Jacques Prandi |
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| Přispěvatelé: | Chemical Biology 2, Department of Engineering Mathematics, University of Bristol, University of Bristol [Bristol], Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, University of Cape Town |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Models
Molecular 0301 basic medicine Acylation T-Lymphocytes [SDV]Life Sciences [q-bio] Clinical Biochemistry Lymphocyte Activation 01 natural sciences Biochemistry Antigens CD1 Drug Discovery Receptor ComputingMilieux_MISCELLANEOUS lipid antigen antigen-presentation 3. Good health Cell biology tuberculosis Molecular Medicine mycobacteria Antigen presentation T cells CD1 Biology Article Cell Line Mycobacterium tuberculosis 03 medical and health sciences Glycolipid Antigen Journal Article Humans Tuberculosis [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology human [SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biochemistry [q-bio.BM] Molecular Biology Pharmacology Antigens Bacterial 010405 organic chemistry T-cell receptor biology.organism_classification 0104 chemical sciences 030104 developmental biology Cell culture Glycolipids Protein Multimerization T cell receptor |
| Zdroj: | Cell Chemical Biology, 25(4), 392-402.e14. CELL PRESS Cell Chemical Biology Cell Chemical Biology, Cell Press, 2018, 25 (4), pp.392-402.e14. ⟨10.1016/j.chembiol.2018.01.006⟩ James, C A, Yu, K K Q, Gilleron, M, Prandi, J, Yedulla, V R, Moleda, Z Z, Diamanti, E, Khan, M, Aggarwal, V K, Reijneveld, J F, Reinink, P, Lenz, S, Emerson, R O, Scriba, T J, Souter, M N T, Godfrey, D I, Pellicci, D G, Moody, D B, Minnaard, A J, Seshadri, C & Van Rhijn, I 2018, ' CD1b tetramers identify T cells that recognize natural and synthetic diacylated sulfoglycolipids from mycobacterium tuberculosis ', Cell Chemical Biology, vol. 25, no. 4, pp. 392-402.e14 . https://doi.org/10.1016/j.chembiol.2018.01.006 |
| ISSN: | 2451-9448 2451-9456 |
| Popis: | Mycobacterial cell wall lipids bind the conserved CD1 family of antigen-presenting molecules and activate T cells via their T cell receptors (TCRs). Sulfoglycolipids (SGLs) are uniquely synthesized by Mycobacterium tuberculosis, but tools to study SGL-specific T cells in humans are lacking. We designed a novel hybrid synthesis of a naturally occurring SGL, generated CD1b tetramers loaded with natural or synthetic SGL analogs, and studied the molecular requirements for TCR binding and T cell activation. Two T cell lines derived using natural SGLs are activated by synthetic analogs independently of lipid chain length and hydroxylation, but differentially by saturation status. By contrast, two T cell lines derived using an unsaturated SGL synthetic analog were not activated by the natural antigen. Our data provide a bioequivalence hierarchy of synthetic SGL analogs and SGL-loaded CD1b tetramers. These reagents can now be applied to large-scale translational studies investigating the diagnostic potential of SGL-specific T cell responses or SGL-based vaccines. Sulfoglycolipids (SGLs) are uniquely synthesized by Mycobacterium tuberculosis (Mtb) and recognized by human T cells. James, Yu et al. describe a new hybrid synthesis for key antigenic determinants of SGLs and the development of SGL-specific tetramers that can now be applied to large-scale translational studies. |
| Databáze: | OpenAIRE |
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