Rapid identification of pathogens in blood culture with fluorescent in situ hybridization (FISH)
| Autor: | Károly Nagy, Marianne Konkoly-Thege, Andrea Horváth, Katalin Kristóf |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Gram-negative bacteria
General Immunology and Microbiology biology medicine.diagnostic_test Bacteria Gram-positive bacteria Bacteremia General Medicine In situ hybridization medicine.disease biology.organism_classification Molecular diagnostics Microbiology Sepsis Blood medicine Humans Blood culture Fungemia In Situ Hybridization Fluorescence Fluorescence in situ hybridization Candida |
| Zdroj: | Acta microbiologica et immunologica Hungarica. 57(3) |
| ISSN: | 1217-8950 |
| Popis: | Rapid identification of pathogens in bloodstream infections is of utmost importance to improve survival of septic patients. Fluorescent in situ hybridization (FISH) accelerates the identification of most frequent bacterial and yeast pathogens of sepsis. In this study, 210 positive blood cultures were tested with FISH method and the results were evaluated comparing to the traditional cultivation based results. Overall agreement between FISH and conventional identification was 91.4%, with better results for Gram-negative bacteria than for Gram-positives (100% and 89.5%, respectively). FISH results were obtained within 1 hour. FISH may serve as a useful tool to supplement traditional microbiological methods for rapid, provisional identification of sepsis pathogens. |
| Databáze: | OpenAIRE |
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