Trypanosoma cruzi: Immune response in mice immunized with parasite antigens
| Autor: | Rita L. Cardoni, Ernesto H. de Titto, Martin E. Rottenberg, Elsa L. Segura, Mirta Moreno |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Male
Bordetella pertussis Trypanosoma cruzi medicine.medical_treatment Immunology Antibodies Protozoan Antigens Protozoan chemical and pharmacologic phenomena Spleen Parasitemia Lymphocyte Activation Microbiology Mice Immune system Adjuvants Immunologic Antigen Microsomes parasitic diseases medicine Animals Chagas Disease Hypersensitivity Delayed Immunity Cellular biology General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification medicine.disease Infectious Diseases medicine.anatomical_structure Immunization Parasitology Adjuvant |
| Zdroj: | Experimental Parasitology. 65:101-108 |
| ISSN: | 0014-4894 |
| DOI: | 10.1016/0014-4894(88)90111-7 |
| Popis: | The humoral and cellular immune responses were studied in mice immunized with flagellar fraction (F), F plus Bordetella pertussis as adjuvant (F-Bp), and microsomal (Mc) subcellular fractions from the epimastigote forms of Trypanosoma cruzi. The immune response was studied before and after the challenge with 50 bloodstream forms of T. cruzi, Tulahuén strain. The immunization with F-Bp, but not with Mc or F and Bp separately, protected mice, in terms of parasitemia and mortality, from the challenge with the parasite. Before the challenge, levels of specific antibodies in mice immunized with F-Bp were higher than in mice immunized with F or Mc. Antibody levels 17 days after the infection were similar in the three groups of mice while nonimmunized mice reached lower levels. Early during the infection nonimmunized infected mice lacked delayed-type hypersensitivity (DTH) responses to parasite antigens and to concanavalin A (Con A). Mice immunized with F-Bp, however, presented positive DTH responses to parasite antigens and Con A both, before and after the challenge with T. cruzi. DTH reaction was transferred with spleen cells. Mice immunized with Mc behaved similarly to infected nonimmunized animals in their reactivity to parasite antigens. These results indicated striking differences between protected and nonprotected mice in humoral and cellular immune responses during experimental T. cruzi infection. |
| Databáze: | OpenAIRE |
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