Urinary metabolomics reveals the therapeutic effect of HuangQi Injections in cisplatin-induced nephrotoxic rats
| Autor: | Wenzheng Ju, Yao-Yao Wan, Shijia Liu, Xuan-Sheng Ding, Hui-Ting Song, Chang-Yin Li, Yue-Heng Liu, Guoliang Dai, Xiao-Xiao Wang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
inorganic chemicals Male Science Antineoplastic Agents Pharmacology Article Nephrotoxicity 03 medical and health sciences chemistry.chemical_compound Tandem Mass Spectrometry medicine Metabolome Animals Metabolomics Purine metabolism Cisplatin Creatinine Multidisciplinary Fatty acid metabolism business.industry Metabolism Rats Disease Models Animal 030104 developmental biology chemistry Toxicity Medicine Kidney Diseases business Biomarkers Metabolic Networks and Pathways medicine.drug Chromatography Liquid Drugs Chinese Herbal |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-12 (2017) |
| ISSN: | 2045-2322 |
| Popis: | The side effects of cisplatin (CDDP), notably nephrotoxicity, greatly limited its use in clinical chemotherapy. HuangQi Injections (HI), a commonly used preparation of the well-known Chinese herbal medicine Astragali radix, appeared to be promising treatment for nephrotoxicity without compromising the anti-tumor activity of CDDP. In this study, the urinary metabolomics approach using liquid chromatography time of flight mass spectrometry (LC-TOF/MS) was developed to assess the toxicity-attenuation effects and corresponding mechanisms of HI on CDDP-exposed rats. As a result, successive administration of HI significantly recovered the decline of body weight and downregulated the abnormal increase of serum creatinine and urea. HI partly restored the CDDP-induced alteration of metabolic profiling back into normal condition. Totally 43 toxicity-attenuation potential biomarkers were screened and tentatively identified, which were involved in important metabolic pathways such as amino acid metabolism, TCA cycle, fatty acid metabolism, vitamin B6 metabolism and purine metabolism. The results clearly revealed that HI could alleviate CDDP-induced nephrotoxicity and improve the disturbed metabolic balance induced by repeated CDDP exposure. The present study provided reliable evidence for the protective effect of HI on CDDP-induced toxicity with the multi-target pharmacological characteristics. |
| Databáze: | OpenAIRE |
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