Renovascular morphological changes in a rabbit model of hydronephrosis
| Autor: | Ren-ping Zheng, Jie Zhang, Lu-sheng Zhang, Jian Yang, Zi-qiang Dong, Xiao-guang Zhou, Wan-qiang Li, Bing Long, Xiao-bing Zhou |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Afferent arterioles Biomedical Engineering Urology Lumen (anatomy) Blood Pressure Hydronephrosis urologic and male genital diseases Biochemistry Biomaterials Renal Artery medicine.artery Glomerular Basement Membrane Genetics medicine Renal fibrosis Animals Renal artery Earth-Surface Processes business.industry Anatomy medicine.disease Obstructive Nephropathy Disease Models Animal medicine.anatomical_structure Blood pressure Rabbits business Artery |
| Zdroj: | Journal of Huazhong University of Science and Technology [Medical Sciences]. 34:575-581 |
| ISSN: | 1993-1352 1672-0733 |
| DOI: | 10.1007/s11596-014-1318-9 |
| Popis: | Obstructive nephropathy ultimately leads to end-stage renal failure. Renovascular lesions are involved in various nephropathies, and most renal diseases have an ischemic component that underlies the resulting renal fibrosis. The aim of this study was to investigate whether morphological changes occur in the renal vasculature in hydronephrosis and the possible mechanisms involved. A model of complete unilateral ureteral obstruction (CUUO) was used. Experimental animals were divided into five groups: a normal control group (N) and groups of animals at 1st week (O1), 2nd week (O2), 4th week (O4) and 8th week (O8) after CUUO. Blood pressure was measured, renal arterial trees and glomeruli were assessed quantitatively, and renovascular three-dimensional reconstruction was performed on all groups. Glomerular ultrastructural changes were examined by transmission electron microscopy. The results showed that the systolic blood pressure was significantly increased in the obstructed groups (O1, O2, O4 and O8). Three-dimensional reconstruction showed sparse arterial trees in the O8 group, and a tortuous and sometimes ruptured glomerular basement membrane was found in the O4 and O8 groups. Furthermore, epithelial media thickness and media/lumen ratio were increased, lumen diameters were decreased, and the cross-sectional area of the media was unaltered in the segmental renal artery, interlobar artery and afferent arterioles, respectively. In conclusion, renal arterial trees and glomeruli were dramatically altered following CUUO and the changes may be partially ascribed to vascular remodeling. Elucidation of the molecular mechanisms of renovascular morphological alterations will enable the development of potential therapeutic approaches for hydronephrosis. |
| Databáze: | OpenAIRE |
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