Seroprevalence of dengue among healthy adults in a rural community in Southern Malaysia: a pilot study

Autor: Pascale Allotey, Nowrozy Kamar Jahan, Cheong Yuet Meng, Anuar Zaini Md Zain, Faidzal Adlee Ghafar, Lau Wee Ming, Hamdan Ahmad, Ahmad Abdul Basitz Ahmad Fatan, Aman Bin Rabu, Amreeta Dhanoa, Mohtar Pungut Ahmad, Quek Kia Fatt, Iekhsan Othman, Sharifah Syed Hassan, Maude E. Phipps, Daniel D. Reidpath, Rowther Sirajudeen
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Male
Rural Population
Seroprevalence
Pilot Projects
Dengue virus
medicine.disease_cause
Antibodies
Viral
Dengue fever
Disease Outbreaks
Dengue
0302 clinical medicine
Residence Characteristics
Seroepidemiologic Studies
Rural
030212 general & internal medicine
Subclinical infection
lcsh:Public aspects of medicine
Age Factors
General Medicine
Middle Aged
Community-based study
Healthy Volunteers
Infectious Diseases
Female
medicine.symptom
Research Article
Adult
030231 tropical medicine
Enzyme-Linked Immunosorbent Assay
Asymptomatic
lcsh:Infectious and parasitic diseases
03 medical and health sciences
medicine
Humans
lcsh:RC109-216
Disease burden
business.industry
Public Health
Environmental and Occupational Health
Malaysia
Outbreak
lcsh:RA1-1270
medicine.disease
Vaccine efficacy
Immunoglobulin M
Immunoglobulin G
business
Demography
Zdroj: Infectious Diseases of Poverty, Vol 7, Iss 1, Pp 1-13 (2018)
Infectious Diseases of Poverty
ISSN: 2049-9957
DOI: 10.1186/s40249-017-0384-1
Popis: Background The frequency and magnitude of dengue epidemics continue to increase exponentially in Malaysia, with a shift in the age range predominance toward adults and an expansion to rural areas. Despite this, information pertaining to the extent of transmission of dengue virus (DENV) in the rural community is lacking. This community-based pilot study was conducted to establish DENV seroprevalence amongst healthy adults in a rural district in Southern Malaysia, and to identify influencing factors. Methods In this study undertaken between April and May 2015, a total of 277 adult participants were recruited from households across three localities in the Sungai Segamat subdistrict in Segamat district. Sera were tested for immunoglobulin G (IgG) (Panbio® Dengue Indirect IgG ELISA/high-titer capture) and immunoglobulin M (IgM) (Panbio®) antibodies. The plaque reduction neutralization test (PRNT) was conducted on random samples of IgG-positive sera for further confirmation. Medical history and a recall of previous history of dengue were collected through interviews, whereas sociodemographic information was obtained from an existing database. Results The overall seroprevalence for DENV infection was 86.6% (240/277) (95% CI: 83–91%). Serological evidence of recent infection (IgM/high-titer capture IgG) was noted in 11.2% (31/277) of participants, whereas there was evidence of past infection in 75.5% (209/277) of participants (indirect IgG minus recent infections). The PRNT assay showed that the detected antibodies were indeed specific to DENV. The multivariate analysis showed that the older age group was significantly associated with past DENV infections. Seropositivity increased with age; 48.5% in the age group of 45 years (P
Databáze: OpenAIRE
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